Sequences from confirmation amplicon


Confirmation sequences for line 308D08 and hit At1g07747 are shown.
Line ID 308D08
Locus At1g07747 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2403771
Primers used for PCR 09tq - 8474, show primer details
   
Sequence Name 76-K116920-0022-308-D08C-09tq
GenBank Accession LN513455 [GenBank]
Sequencing primer gene specific primer, 09tq: 5'-AACACACACATCAGCCTATACTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K116920-0022-308-D08C-09tq
AAATTAGATAGAAGGAGAAACTTAAGGTTACAACTTACAAGACACGAAGATGAAGTTCAC
TACATTAGTTTTCGTTGCGTTTGTTCTCTCGTCAATGGCTCCGACCAAAGCAGAGGAGAA
AGCGGCATGCGTCGTGACAGATCTCAAAGCATGCCTACCAGATATAATAGGCGAAACCCC
ACCATCGACAAAATGTTGCACAAAATTGAAAGATAAAAAGTCGTGTTTTTGTGAGTACTT
GAAAAACCCATTGATTGCTCCATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 32-K116919-0022-308-D08C-8409
GenBank Accession LN500733 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116919-0022-308-D08C-8409
CAATCAATGGGTTTTTTCAAGTACTCACAAAAACACGACTTTTTATCTTTCAATTTTGTG
CAACATTTTGTCGATGGTGGGGTTTCGCCTATTATATCTGGTAGGCATGCTTTGAGATCT
GTCACGACGCATGCCGCTTTCTCCTCTGCTTTGGTCGGAGCCATTGACGAGAGAACAAAC
GCAACGAAAACTAATGTAGTGAACTTCATCTTCATGTCTTGTAAGTTGTAACCTTAAGTT
TCTCCTTCTATCTAATTTCTAGTCTTGCTTAGAAAATGCTTGAAATAATCGATTACGAGA
TTTGTTTAAGAATGGAGTAATGTTTTGCAGTATAGGCTATTTTTGTGTGTT

Last Updated on 06.12.2018 16:11