Sequences from confirmation amplicon


Confirmation sequences for line 308F01 and hit At5g22380 are shown.
Line ID 308F01
Locus At5g22380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7409824
Primers used for PCR YI03 - 8474, show primer details
   
Sequence Name 45-K027346-022-308-F01C-8409
GenBank Accession LN505403 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K027346-022-308-F01C-8409
GCCATTTACAATTGAATATATATATTCATATATATGCCATAGTAGATATATATATCGACA
ATTCAGGTGAGGTTAACCGTTAACGTACTTGGAAGATGACTAGGCTCGACCTCAAAGACG
TCAAGTACGGGAATGACACGATGCATTGAGTCATCACTCCTTCCTTCGAGCTGGTTTCGT
AGGTAGAACGAAACCAGTTCTTCTTCCGTGGGATAGAAGCGAAACCCGATTGTGACCTCA
TCGGCCATTTTTGTACGATATTTTTAACAACAAGAAACACTCTAATTAGTTGTAGCTACC
TATATTATTAACTTAGATGCCATGGATTCATTGTCATTAAATAAGAAGTAGAGATTGGTT
TCTCCGAGTGAAAGGAGAAAAGTTCTCTACGCGTTTTTCGTTTTTTTTTTTTATTTTATT
CCCTTTTTGTACGGATATTGGTAAAAACAAGGATGTTTTGGGGGGGGGTGTTTGATTACT
TTCGTTGTTATGGGTTTACGGTTTAGAAAACTTTTTACAAGCCACGTACGTCCATTGAAT
GAGTCAAGCATTCGTCTTTGCCGTCAAGTTTCAGTCTCTTTTCCTTTAGTTTTCCAATCT
TTTAA
   
Sequence Name 46-K027346-022-308-F01C-YI03
GenBank Accession LN505745 [GenBank]
Sequencing primer gene specific primer, YI03: 5'-CTGGCAAGCCAAATTAAAAGATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K027346-022-308-F01C-YI03
ACTTGACGGCAAGACGAATGCTTGACTCATTCAATGGACGTACGTGGCTCGTAAAAAGTT
TACTAAACAGTAAACACATAACAACGAAAGTAATCAAACACACACACACAAAACATCCTT
GTCTTTACCAATATCCGTACAAAAAGGGAATAAAATAAAAAAAAAAAACGAAAAACGCGT
AAAGAACTTTTCCCCTTTCACTCGGAAAAACCAATCTCTACTTCTTATTTAAGGACAATG
AATCCATGGCATCTAAGTTAATAATATAGGTAGCTACAACTAATTAGAGGGTTTCTGGTT
GTTAAAAATATCGTACAAAAATGGCCGATGAGGTCACAATCGGGTTTCGCTTCTATCCCA
CGGAAAAAAAACTGGTTTCGTTCTACCTACAAAACCAGCTCGAAGGAAGGAGTGATGACT
CAATGCATCGGGTCATTCCCGTACTTGACGTCTTTGAGGTCGAGCCTAGTCATCTTCCAA
GTACGTTAACGGTTAACCTCACCTGAATTGTCGATATATATATCTACTATGGCATATATT
TGAATATATATTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAAATGTCCGCAGCGTTATTATAAAATGAAAGTACTTTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATGGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATT

Last Updated on 06.12.2018 16:11