Sequences from confirmation amplicon


Confirmation sequences for line 309E12 and hit At5g07210 are shown.
Line ID 309E12
Locus At5g07210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2253967
Primers used for PCR MI03 - 8474, show primer details
   
Sequence Name 27-K018320-022-309-E12C-MI03
GenBank Accession LN498171 [GenBank]
Sequencing primer gene specific primer, MI03: 5'-TAAGCAACAAGAGGAACGGTAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K018320-022-309-E12C-MI03
CACAATGCACGCAGATGTTAGTGTCCCTCAGCAAATCACGTTGGTTCCTGAGCAAGCTGA
CGTCTTAAAGACCAAGAGGAAGAACTGCTCATTTAAATCGGATTCAAGAACCGTGAACAG
TACAAATGGAAGTTGTGTTAGTACGGATGGTTCAAGAAAAAATCGAAAAAGAAAACCCAA
CGGTGGTCCTAGTGATGATGGCGAGTCTATGTCGCAACCCGCGAAAAAGAAAAAGATTCA
GTGGACGGATTCTCTTCACGACCTATTCTTACAGGCTATCCGACATATTGGTCTTGATAG
TAAGTGTTTTGATTTTCTTAAAAAAAACGATATATATATAGTACATTACTGTGCTATATA
TTCAATTGTTTTTTGAGTTGCTATATATTACACAAATTCCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
   
Sequence Name 31-K018320-022-309-E12C-8409
GenBank Accession LN499953 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K018320-022-309-E12C-8409
AGATTTCTCGAACATGAAGCCATTTACAATTGAATATATCCTGGAATTTGTGTAATATAT
AGCAACTCAAAAAACAATTGAATATATAGCACAGTAATGTACTATATATATATCGTTTTT
TTTAAGAAAATCAAAACACTTACTATCAAGACCAATATGTCGGATAGCCTGTAAGAATAG
GTCGTGAAGAGAATCCGTCCACTGAATCTTTTTCTTTTTCGCGGGTTGCGACATAGACTC
GCCATCATCACTAGGACCACCGTTGGGTTTTCTTTTTCGATTTTTTCTTGAACCATCCGT
ACTAACACAACTTCCATTTGTACTGTTCACGGTTCTTGAATCCGATTTAAATGAGCAGTT
CTTCCTCTTGGTCTTTAAGACGTCAGCTTGCTCAGGAACCAACGTGATTTGCTGAGGGAC
ACTAACATCTGCGTCCTTGTGGTTTTGCTCGGTCGATAGTGTTGACTTACCGTTCCTC

Last Updated on 06.12.2018 16:11