Sequences from confirmation amplicon


Confirmation sequences for line 309F04 and hit At1g68905 are shown.
Line ID 309F04
Locus At1g68905 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25906820
Primers used for PCR 23ns - 8474, show primer details
   
Sequence Name 43-K114626-0022-309-F04C-23ns
GenBank Accession LN504929 [GenBank]
Sequencing primer gene specific primer, 23ns: 5'-AGTCACATACCAGTTTTGGCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114626-0022-309-F04C-23ns
TCTTGGCAAAGCATTGAATCTTGTATTTATCTCAAACAATGCATGTATGTGGTTTATTAA
TTCCCTCTACAGATACAAATTCAACAAACTATCAAATGATTTCCTAAAATGGAGAAATTT
CACAAGTTGGATTAAAACAATGTGTAATCAAACTTGGTATATAAAAGAAGACAATCTGAC
TTCTCTCTTCCTAACTTGTACATTCAAAATAAACAAGAAAAGACTTTTGAGATTACATTA
ACTAAGCAATGGAGAAAACGTCTCTCAAGCTTATCTTCTTGTTCTCCCTCACAGTCATAG
CATTTTGTAAGCTTCTTCTACATGATAAGTTCTTCTTTATATAATGTTTTGTTTTGGTCT
TGTTTGACGTTTATGGTGTGTATTTGTTGATATAGGTTCATCTTTAGGTGATGCTAGAGA
GATGGTGAAAGCAGAAGTGAATTGCATCGGCGGCAAATGTCCTGAAGGAAAGAAGAACTG
TAATTGCATGCCACCGATAGCACATGTGATGGATGATATCATGTCCATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 43-K114625-0022-309-F04C-8409
GenBank Accession LN504928 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K114625-0022-309-F04C-8409
TTGAATGGACATGATATCATCCATCACATGTGCTATCGGTGGCATGCAATTACAGTTCTT
CTTTCCTTCAGGACATTTGCCGCCGATGCAATTCACTTCTGCTTTCACCATCTCTCTAGC
ATCACCTAAAGATGAACCTATATCAACAAATACACACCATAAACGTCAAACAAGACCAAA
ACAAAACATTATATAAAGAAGAACTTATCATGTAGAAGAAGCTTACAAAATGCTATGACT
GTGAGGGAGAACAAGAAGATAAGCTTGAGAGACGTTTTCTCCATTGCTTAGTTAATGTAA
TCTCAAAAGTCTTTTCTTGTTTATTTTGAATGTACAAGTTAGGAAGAGAGAAGTCAGATT
GTCTTCTTTTATATACCAAGTTTGATTACACATTGTTTTAATCCAACTTGTGAAATTTCT
CCATTTTAGGAAATCATTTGATAGTTTGTTGAATTTGTATCTGTAGAGGGAATTAATAAA
CCACATACATGCATTGTTTGAGATAAATACAAGATTCAATGCTATTGCCAAGAGAATCCA
AAGACATTTTAGAATTGAAGATATATTTCCTTTAACAAAAGCCAAA

Last Updated on 06.12.2018 16:11