Sequences from confirmation amplicon


Confirmation sequences for line 309F08 and hit At3g27570 are shown.
Line ID 309F08
Locus At3g27570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10215277
Primers used for PCR LG04 - 8474, show primer details
   
Sequence Name 65-K030473-022-309-F08C-8409
GenBank Accession LN511315 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K030473-022-309-F08C-8409
ATTGAATATGACGATCATTGGTAAGAAAATGTCAAATTCACCTACTCATATATAATTCAG
AAAAGTAATTGAATGTAATATATTATCGCATAACGATCTTGGTTTTATGCAGGTATGGCT
ATGTGACTCCTGATGACGTGCCTGCAATGCTTGATCAGCATATTGCAAAAGGAGAAATCA
TTCAAAACCTTTCCAGGTCTGTTTTCCAACTTCTTGTTTCTAGATTGTTGCAGATATGTC
TCTGTTAAAACAGCTTTACATTTCCTCGTGTACATTGTACTTTTTATATCGATATCCATG
GTTGTAGTAAACCTTACACTAGACATATACCCTCGTGTTTGAACTGAAACGGTTTTTCAA
CAGACAAAAACTATTGTTTGCTAATGAATGTTGCAACTCCGTATATGTATAACTAGTATG
ATACTGCAATGTAGTCAAGAAGTTTCTGCGTGTAATGGTACTTTCGCTGAGGTTTTTGTT
TGCCCAACTAGGAAGCTCATAACCCTTTACTCACCCATTTTATCTAATAAAATATTGTTA
TCACTTTATTTCTAGAACCATAGTCAACAAGTTTCAGGTTCATACCTTGTCTGATTGCTT
ATCTTCATTTAACGCT
   
Sequence Name 66-K030473-022-309-F08C-LG04
GenBank Accession LN511517 [GenBank]
Sequencing primer gene specific primer, LG04: 5'-TTGAAAACACAGCGTTAAATGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K030473-022-309-F08C-LG04
AAGGGTTCCGAGCTTTCTAGTTGGGCAAAACAAAAACCTTCAGCGAAAGTACCATTACAC
GCAGAAAACTTCTCGACTACATCGCAGTATCATACTAGTTATACATATACGGAGTTCGCA
ACATTCATTAGCAAACAATAGTTTTTGTCTGTTGAAAAACCGTTTCAGTTCAAACACGAG
GGTATATGTCTAGTGTAAGGTTTACTACAACCATGGATATCGATATAAAAAGTACAATGT
ACACGAGGAAATGTAAAGCTGTTTTAACAGAGACATATCTGCAACAATCTAGAAACAAGA
AGTTGGAAAACAGACCTGGAAAGGTTTTGAATGATTTCTCCTTTTGCAATATGCTGATCA
AGCATTGCAGGCACGTCATCAGGAGTCACATAGCCATACCTGCATAAAACCAAGATCGTT
ATGCATAATATATTACATTCAATTACTTTTCTGAATTATATATGCAAGTTGAATTTGACA
TTTTCTTACCAATGATCGTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTNTTCATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATNTCGACGT
Primers used for PCR 59wd - 8474, show primer details
   
Sequence Name 24-K118209-0022-309-F08C-59wd
GenBank Accession LN497179 [GenBank]
Sequencing primer gene specific primer, 59wd: 5'-TTCTTTGCGTTGTTATGGATACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118209-0022-309-F08C-59wd
CTAGTTTTGCCTCCATTTTTCTCTTTAGGGAAAGATTTTGACTAGGACTTGACCTCATTG
TTTCGAATTCTTGCTTCGTATTTCGATTTCTAGGGATTTAAGAACTGTAGATTCTCAGAT
TTTGTGTCTTTAAGTTGTAGAGATGATGATGTTTAAACTCAAGTTTTGGATCTGAAAAGC
CGATTTCCGGTCATTCTGTTAATATGTGTTTATGTTAGTTACTCTACGTTGTTTTGGATC
CTGTAGCTATCTTGTTATTTATCCTTGTACATTTTCTATTCTTGTTGTTATTGTTAAAGG
GCGATTCAGGACACAGATGTGGATGCTTTTGTGGAAGATGTGCTTGTGAAAGGAAAAACA
TGGACTTCTGGGATTCAGGAAGAGTTGACTGGTTCCTTTGTGTTTGTGTGCGCTCATGGT
AGCCGAGACAAGAGATGTGGTGTTTGTGGACCAGTTCTTATGGAGAAGTTCGAGCAGGAG
ATAAGTTCACGTGGACTTTCAGACAAAATCTTTGTTTTGCCGTGTTCTCATATCGGTGGG
CACAAGTATGCTGGAAATTTGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGT
   
Sequence Name 12-K118207-0022-309-F08C-8409
GenBank Accession LN491786 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118207-0022-309-F08C-8409
TTTACATCAAATTTCCAGCATACTTGTGCCCACCGATATGAGAACACGGCAAAACAAAGA
TTTTGTCTGAAAGTCCACGTGAACTTATCTCCTGCTCGAACTTCTCCATAAGAACTGGTC
CACAAACACCACATCTCTTGTCTCGGCTACCATGAGCGCACACAAACACAAAGGAACCAG
TCAACTCTTCCTGAATCCCAGAAGTCCATGTTTTTCCTTTCACAAGCACATCTTCCACAA
AAGCATCCACATCTGTGTCCTGAATCGCCCTTTAACAATAACAACAAGAATAGAAAATGT
ACAAGGATAAATAACAAGATAGCTACAGGATCCAAAACAACGTAGAGTAACTAACATAAA
CACATATTAACAGAATGACCGGAAATCGGCTTTTCAGATCCAAAACTTGAGTTTAAACAT
CATCATCTCTACAACTTAAAGACACAAAATCTGAGAATCTACAGTTCTTAAATCCCTAGA
AATCGAAATACGAAGCAAGAATTCGAAACAATGAGGTCAAGTCCTAGTCAAAATCTTTCC
CTAAAGAGAAAAATGGAGGCAAAACTAGCTGAGAATCGAATCTCTGAGGGCACAAGGATA
AGTTAGAAGATAGCTACAGTATCCAT

Last Updated on 06.12.2018 16:11