Sequences from confirmation amplicon


Confirmation sequences for line 309G05 and hit At1g67360 are shown.
Line ID 309G05
Locus At1g67360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25237783
Primers used for PCR IN28 - 8474, show primer details
   
Sequence Name 43-K033543-022-309-G05C-8409
GenBank Accession LN504746 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K033543-022-309-G05C-8409
TAACATGTAGTTACTCTTTCTTTCGTTATCAGACAAAGCTTTGCCTGTGGCTGCACACTT
TTCCAGTCGGTACAACGATTTGGTGACTGATATGACTAATATGGGTTACTCTTTGGTTTG
GTTA
   
Sequence Name 43-K033543-022-309-G05C-IN28
GenBank Accession LN504747 [GenBank]
Sequencing primer gene specific primer, IN28: 5'-GATAACCAACCAAAGAGTAACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K033543-022-309-G05C-IN28
GACTGGAAAGTGTGCAGCCACAGGCAAAGCTTTGTCACCCATTGCATGGATTGGTTTATA
CTGGTTAAGTTTAGCCCAGACTTTAACCGAGTTGGTCACAACGAAAAACTTGTACTCAGT
TGCAGCATAGTTAAACGCAGCTTTGGGACCACCGGTACGAGCCTCTTTCACAAAGCTTTG
AGCTTTCTCTGTCGCCTTGTAGATCAATACATGTGCTTGATTCACCACTTTCTTAGCCAT
TGGAGGAGCATGCTCATCAAACTTGTACGAAACTTCACCCACCTAGCAAAAGTAAATAAA
GAAAGGAAATGTTTGAAAAACTAAACACGATCTGGTTTCTAAATTAGGTCATCAGATCCA
ACAAAGACTAAATCTATGAGAAATTCAAAATTGGGCTCTACCTTGTGATCTAGAAAGACC
AGGAGAGAATCAGGAACATCTTTGAATTTCTGATAGACAGGGGTGACGACGGTGGTGACG
GCTCCTTCGACCTTTTCCACGGCGGATTTGATTCTTGTACGAACTATTCTTGTGTAGACG
GGATAGAATCCATTGGAATTGGTTTCAAGGCAGGCATATCTACATGGATAATCAATGACT
CTGACGGG

Last Updated on 06.12.2018 16:11