Sequences from confirmation amplicon


Confirmation sequences for line 310C05 and hit At4g31490 are shown.
Line ID 310C05
Locus At4g31490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15270705
Primers used for PCR 54yx - 8474, show primer details
   
Sequence Name 50-K123255-0022-310-C05C-54yx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 54yx: 5'-AAAAACTAAACAGAGTGCGAAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K123255-0022-310-C05C-54yx
ATAAGCGAGGGAAGCTTAGAAATAATAATATTACCTTCCGACTCTTTAGATGGAAGAAGT
CAATTAGATCATCCGGTTGAGCATGAGTTGTTTGGGTCTTTGCCTTCAGTTCCTCCATCT
CTCTAAGCTGTTTTTCAGAAATCATCTTGACAAAACTCTGGCGGCAGGATTCCAACCATA
TCTTTTTCATCTCAACATTCCTATGGCAAAGCAATTTTATGCACAACATAATCCGCTCAT
ACGAATCATTATCAATAGGGTGTGGAGAAACAGGAGATTGCCCAAGTTGCAAAATAGAAA
CCATGATTAGCAAAGCCTGTGATACTGTCTTGTTTACTTCAGTTTTGGAAGACTGAACCT
CTTCCAACCTAAGAACAAGTTTGGTCAACGTGCAAGCAACCACAGCTCCGAGGAAAAAAT
CACCAGTTAGAAGGAGTGCCCTCAAATTTCCAGAAGTCAGTGATCCTTGAACAACTGTAG
GCGAGGAGAATGTGGTTTCAGAGGCTGCGCTTTGTGTAGCATAAGTTCCATCAGCAAGAA
TAACTGGCTTTCTAGAGGACACCATGGCAGAAGAGGTAGGCTGAATCTTCTTTGATGTCT
CAGTTGGCTCAGACTCCTCAGAAACAGAGTAAAATGGTAATTCGCCAAGGCATTGTGTAA
TAGTTGAAATGCCACTCTCAACTTCTGAAAGTGATAGGCAATACTCACCAATGATCCAAA
GTGCACAAGGGCAGACCTTTCCTGCACGGATCTGATAGAACGTGTCCAACAACCTGGTGA
TGATTGAAACTCTCAACTTGGGATTTGTTTCTATTATCTCTCTAACGAAAACAACAACCA
TGTAGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCCATTTTTTTTCAATAAAAAAA
TGT
   
Sequence Name 18-K123254-0022-310-C05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K123254-0022-310-C05C-8409
CATTTACAATTGAATATCTACATGGTTGTTGTTTTCGTTAGAGAGATAATAGAAACAAAT
CCCAAGTTGAGAGTTTCAATCATCACCAGGTTGTTGGACACGTTCTATCAGATCCGTGCA
GGAAAGGTCTGCCCTTGTGCACTTTGGATCATTGGTGAGTATTGCCTATCACTTTCAGAA
GTTGAGAGTGGCATTTCAACTATTACACAATGCCTTGGCGAATTACCATTTTACTCTGTT
TCTGAGGAGTCTGAGCCAACTGAGACATCAAAGAAGATTCAGCCTACCTCTTCTGCCATG
GTGTCCTCTAGAAAGCCAGTTATTCTTGCTGATGGAACTTATGCTACACAAAGCGCAGCC
TCTGAAACCACATTCTCCTCGCCTACAGTTGTTCAAGGATCACTGACTTCTGGAAATTTG
AGGGCACTCCTTCTAACTGGTGATTTTTTCCTCGGAGCTGTGGTTGCTTGCACGTTGACC
AAACTTGTTCTTAGGTTGGAAGAGGTTCAGTCTTCCAAAACTGAAGTAAACAAGACAGTA
TCACAGGCTTTGCTAATCATGGTTTCTATTTTGCAACTTGGGCAATCTCCTGTTTCTCCA
CACCCTATTGATAATGATTCGTATGAGCGGATTATGTTGTGCATAAAATTGCTTTGCCAT
AGGAATGTTGAGATGAAAAAGATATGGTTGGAATCCTGCCGCCAGAGTTTTGTCAAGATG
ATTTCTGAAAAACAGCTTAGAGAGATGGAGGAACTGAAGGCAAAGACCCAAACAACTCAT
GCTCAACCGGATGATCTAATTGACTTCTTCCATCTAAAGAGTCGGAAGGTAATATTATTA
TTTTCTAAGCTTTCCCTCGCTTTATTGTGTGTTCTTTTCCTTATGATT

Last Updated on 06.12.2018 16:11