Sequences from confirmation amplicon


Confirmation sequences for line 310H12 and hit At2g29740 are shown.
Line ID 310H12
Locus At2g29740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12707986
Primers used for PCR ZZ67 - 8474, show primer details
   
Sequence Name 31-K118804-0022-310-H12C-ZZ67
GenBank Accession LN500354 [GenBank]
Sequencing primer gene specific primer, ZZ67: 5'-ATTAAAGAGATCGCTCAAGCCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K118804-0022-310-H12C-ZZ67
GACGGACCCGAAGAGTACGCGAGCCCGAACGAGATTTTACCGGACGGGTTTATGAACCGA
GTCATGGGTTTGGGCCTTGTTTGTGGTTGGGCTCCTCAAGTTGAAATTCTGGCCCATAAA
GCAATTGGAGGGTTCGTGTCACACTGCGGTTGGAACTCGATATTGGAGAGTTTGCGTTTC
GGAGTTCCAATTGCCACGTGGCCAATGTACGCGGAACAACAACTAAACGCGTTCACGATT
GTGAAGGAGCTTGGTTTGGCGTTGGAGATGCGGTTAGTCGGCTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACA
   
Sequence Name 12-K118803-0022-310-H12C-8409
GenBank Accession LN491806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K118803-0022-310-H12C-8409
ATTTCATTGAGCCGACTAACCGCATCTCCACGCCAAACCAAGCTCCTTCACAATCGTGAA
CGCGTTTAGTTGTTGTTCCGCGTACATTGGCCACGTGGCAATTGGAACTCCGAAACGCAA
ACTCTCCAATATCGAGTTCCAACCGCAGTGTGACACGAACCCTCCAATTGCTTTATGGGC
CAGAATTTCAACTTGAGGAGCCCAACCACAAACAAGGCCCAAACCCATGACTCGGTTCAT
AAACCCGTCCGGTAAAATCTCGTTCGGGCTCGCGTACTCCTTCGGGTCCGTTCGAATCGA
CCAGAGGAATCTGATTCCGACGAGCTCTAAGGCTTGAGCGACCTCTTTAA
Primers used for PCR 61xh - 3144, show primer details
   
Sequence Name 19-K119080-0022-310-H12C-61xh
GenBank Accession LN494984 [GenBank]
Sequencing primer gene specific primer, 61xh: 5'-TGAAATCATAAACGAATAACTTTAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K119080-0022-310-H12C-61xh
ACATAATGATCTCTTTGCATCACATCTCTTCATTAGTCAGTGTCTACAAGTTGATATTGT
GTGTTGTGTCGCACTCACTTCATATTTCAATTCATCAAAGCATGCTTCCTAGTAATACAC
AATGTTAGGGGAGTAGAGATTTAACCATACATATAGTAAGATAGTTGTTTTGTTTTCATT
TTATTGATTAATTTGGGCATCTCAATAGCAGGATAGTAACCCTAATAACTAATTTATCTC
GATTTTACATTTTTGTAATGCTCAATTATACTGTTTTGATGTCTTTTAAAATTCATTTGT
ATTTTGGTTATTTCATATTTCCACAAGTTATATATAAAACAAAATGCAAAATGAGCTATT
TAGATATATAACAAATGAGTAAACACAAAAGATCATGCATACTATCAAAAGGAGGACTTT
TATAGAGTTGGATAAAGCTCAAAGCCATTAGAGACTCACTCAAAGCAAGCTTAAATTACA
TAAAACGACAACGTTTGCATGGTGTAAAAACTTTAAAACCCATCACCGATCAAAGCCCAT
CTATGAATCTTTTAACCGCAACAAACAAAAATCCACCGTCCATCACAGCCTCTTTTCCCG
CCTCCGCAATCTCCTTCAGTTTCCTCCTCGGCACATCCTCACCGTCCATCAAAGATCGTA
CGGCTCCTGCGATTTCATCAGCTTTCACGATTTCTCCATATTCCGACACGTAATCAAACA
CTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTT
   
Sequence Name 05-K119079-0022-310-H12C-35St
GenBank Accession LN488574 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K119079-0022-310-H12C-35St
CTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATTACGTGTCGG
AATATGGAGAAATCGTGAAAGCTGATGAAATCGCAGGAGCCGTACGATCTTTGATGGACG
GTGAGGATGTGCCGAGGAGGAAACTGAAGGAGATTGCGGAGGCGGGAAAAGAGGCTGTGA
TGGACGGTGGATCTTCGTTTGTTGCGGTTAAAAGATTCATAGATGGGCTTTGATCGGTGA
TGGGTTTTAAAGTTTTTACACCATGCAAACGTTGTCGTTTTATGTAATTTAAGCTTGCTT
TGAGTGAGTCTCTAATGGCTTTGAGCTTTATCCAACTCTATAAAAGTCCTCCTTTTGATA
GTATGCATGATCTTTTGTGTTTACTCATTTGTTATATATCTAAATAGCTCATTTTGCATT
TTGTTTTATATATAACTTGTGGAAATATGAAATAACCAAAATACAAATGAATTCTAAAAG
ACATCAAAACAGTATAATTGAGCATTACAAAAATGTAAAATCGAGATAAATTAGTTATTA
GGGTTACTATCCTGCTATTGAGATGCCCAAATTAATCAATAAAATGAAAACAAAACAACT
ATCTAACTATATGTATGGTTAAATCTCTACTCCCCTAACATTGTGTATTACTAGGAAGCA
TGCTTTGATGAATTGAAATATGAAGTGAGTGCGACACAACACACAATATCAACTCGTAGA
CACTGACTAATGAAGAGATGTGATGCAAAGAGATCATTATGTTTATCTCTTCGTAA

Last Updated on 06.12.2018 16:11