Sequences from confirmation amplicon


Confirmation sequences for line 311B01 and hit At2g28760 are shown.
Line ID 311B01
Locus At2g28760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12338135
Primers used for PCR MP28 - 8474, show primer details
   
Sequence Name 39-K020436-022-311-B01C-8409
GenBank Accession LN503203 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K020436-022-311-B01C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATCAAAAGACAATCTCAAGAAATG
GATTGGTCATCCAAGATTTGAGCTAATTCGTCATGGTATAGTATCTTCTTAATTAATCAA
ACTTACTTAATACTCATTAACCAAAACAATGCATTAATACTCATTCTTGTGATTTTTTCA
GATGTGACTGAACCTCTTTTCGTCGAGGTTGATCAAATCTACCATTTAGCATGTCCCGCT
TCTCCAATTTTCTACAAATACAACCCCGTTAAGGTTTGTTAAACTTCGTTAATCAAAACT
CGCAAAATGCTAGCTGATATATTTTTATGACTTATGATCATATATCCTTTTCATCTTTCT
AATGGCAGACGATAAAAACAAATGTGATTGGCACACTTAACATGTTAGGACTAGCTAAGC
GAGTTGGAGCAAGAATTTTACTTACTTCCACATCTGAAGTGTATGGTGATCCTTTGGTTC
ACCCTCAAACCGAGAGCTATTGGGGAAATGTAAACCCGATCG
   
Sequence Name 40-K020436-022-311-B01C-MP28
GenBank Accession LN503559 [GenBank]
Sequencing primer gene specific primer, MP28: 5'-ATAATGACTTACCGATCGGGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K020436-022-311-B01C-MP28
TTTCCCCAATAGCTCTCGGTTTGAGGGTGAACCAAAGGATCACCATACACTTCAGATGTG
GAAGTAAGTAAAATTCTTGCTCCAACTCGCTTAGCTAGTCCTAACATGTTAAGTGTGCCA
ATCACATTTGTTTTTATCGTCTGCCATTAGAAAGATGAAAAGGATATATGATCATAAGTC
ATAAAAATATATCAGCTAGCATTTTGCGAGTTTTGATTAACGAAGTTTAACAAACCTTAA
CGGGGTTGTATTTGTAGAAAATTGGAGAAGCGGGACATGCTAAATGGTAGATTTGATCAA
CCTCGACGAAAAGAGGTTCAGTCACATCTGAAAAAATCACAAGAATGAGTATTAATGCAT
TGTTTTGGTTAATGAGTATTAAGTAAGTTTGATTAATTAAGAAGATACTATACCATGACG
AATTAGCTCAAATCTTGGATGACCAATCCATTTCTTGAGATTGTCTTTTGATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT

Last Updated on 06.12.2018 16:11