Sequences from confirmation amplicon


Confirmation sequences for line 311B09 and hit At3g53750 are shown.
Line ID 311B09
Locus At3g53750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19917106
Primers used for PCR 06ie - 8474, show primer details
   
Sequence Name 52-K112758-0022-311-B09C-06ie
GenBank Accession LN507868 [GenBank]
Sequencing primer gene specific primer, 06ie: 5'-GAGAAGCTTTGCTACATTGCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K112758-0022-311-B09C-06ie
AACAGCTCTTCTGTGAGAAGAACTATGAGTTACCTGATGGGCAAGTGATCACCATTGGAT
CTGAGCGTTTCCGTTGCCCTGAGGTTCTTTACCAGCCATCTATGATCGGTATGGAGAATG
CTGGTATCCATGAAACCACCTACAACTCCATAATGAAATGTGATGTTGACATCAGAAAGG
ACTTGTATGGTAACATTGTGCTCAGTGGTGGAACAACTATGTTCCCTGGAATTGCTGACA
GAATGAGCAAAGAGATCACTGCCATTAGAATAATCGTGAAAAAGAAAGAGTAATCAATAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 12-K112757-0022-311-B09C-8409
GenBank Accession LN491651 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K112757-0022-311-B09C-8409
ATTGATACTCTTTCTTTTTCACGATTATTCTAATGGCAGTGATCTCTTTGCTCATTCTGT
CAGCAATTCCAGGGAACATAGTTGTTCCACCACTGAGCACAATGTTACCATACAAGTCCT
TTCTGATGTCAACATCACATTTCATTATGGAGTTGTAGGTGGTTTCATGGATACCAGCAT
TCTCCATACCGATCATAGATGGCTGGTAAAGAACCTCAGGGCAACGGAAACGCTCAGATC
CAATGGTGATCACTTGCCCATCAGGTAACTCATAGTTCTTCTCTACAGAAGAGCTGGTTT
TAGCCGTCTCAAGCTCCTGCTCATAGTCAAGAGCAATGTAGCNAAGCTTCT

Last Updated on 06.12.2018 16:11