Sequences from confirmation amplicon


Confirmation sequences for line 311E11 and hit At1g17260 are shown.
Line ID 311E11
Locus At1g17260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5904431
Primers used for PCR 04pr - 8474, show primer details
   
Sequence Name 50-K115187-0022-311-E11C-04pr
GenBank Accession LN507268 [GenBank]
Sequencing primer gene specific primer, 04pr: 5'-GGAATTGATTTGGTAAATGTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115187-0022-311-E11C-04pr
TGGCAAAAGGGTTTTGACCCTTCTTGAGCTTGACAGGGTATATTGCCGTTGGAGGAGGTT
TTTGAATACCTAAGAACATCGCCTCAAGGGCTTTTATCTGGAGATGCTGAAGAGAGATTG
AAGATATTTGGTCCTAACAGACTTGAAGAGAAACAGGTAATAAACATGACATTCTTCTTA
GTGTTCCTGCCAATTGTCTCTATTATCTTGTGCTAACCGCCATTTAAAACAATGAGTATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGGTTATTATA
   
Sequence Name 14-K115186-0022-311-E11C-8409
GenBank Accession LN492634 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115186-0022-311-E11C-8409
ATTGAATATATACTCATTGTTTTAATGGCGGTTAGCACAAGATAATAGAGACAATTGGCA
GGAACACTAAGAAGAATGTCATGTTTATTACCTGTTTCTCTTCAAGTCTGTTAGGACCAA
ATATCTTCAATCTCTCTTCAGCATCTCCAGATAAAAGCCCTTGAGGCGATGTTCTTAGGT
ATTCAAAAACCTCCTCCAACGGCAATATACCCTGTCAAGCTCAAGAAGGGTCAAAACCCT
TTTTTGCCACTAAGAATTCAAGTACTAATAAGAGTATACAGTTAAAAGGAACATTTACCA
AATCAATTC
Primers used for PCR 45ve - 3144, show primer details
   
Sequence Name 35-K117497-0022-311-E11C-45ve
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 45ve: 5'-ATCACCAGGTACCAAGATAGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117497-0022-311-E11C-45ve
GAGGGGCCTCCTGCACTGGATCCTTCAAGCCGAAGGCCGGAATCGACACTCTGATCCCTC
GGTAATCAGCTCCTCTCTAAATG
   
Sequence Name 03-K117496-0022-311-E11C-3144
GenBank Accession LN487661 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117496-0022-311-E11C-3144
CTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAATTCTTAGG
TTTTATGTGGAATCCCTTGTCATGGGTTATGGAAGCTGCTGCATTGATGGCCATTGCCCT
CGCTAATAGTCAAGTAAGACAACCACAGTTTTGCTACCATTTTCCATCCATGAGTGTTTA
TGAAAAAGCACCTTGAAACTCATGGTGAAAATGGTATTACAGAGTCTAGGTCCTGACTGG
GAAGACTTTACTGGAATCGTTTGCCTTTTGCTGATCAACGCAACAATCAGCTTCTTTGAA
GAAAACAATGCTGGGAATGCTGCTGCAGCTCTTATGGCTCGCTTGGCTTTAAAAACAAGA
GTATGGCTCTTAACCTTCCTATGAGCCGAAACGGCAAAGTTAGCTGCACTTTAATTTCTA
TATTACCTTAAATTTGATTTAGATCCACTCTTATAGGTTCTTAGAGATGGACAGTGGCAA
GAACAAGATGCTTCTATCTT

Last Updated on 06.12.2018 16:11