Sequences from confirmation amplicon


Confirmation sequences for line 312C11 and hit At4g00750 are shown.
Line ID 312C11
Locus At4g00750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:317236
Primers used for PCR JK42 - 8474, show primer details
   
Sequence Name 40-K100939-0022-312-C11C-8409
GenBank Accession LN503641 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K100939-0022-312-C11C-8409
CCATTTACATTGAATATATCATGAAATCGTATGTCCTTGGGTAAGTTGACATTGCTTCAC
ACCTGTTAAGAGTTTAAGAAATAAAAAAGAATCAATGAATGCTTCCCAAAAAAATGTGTA
TTCGGTTATTAGGAATAGTAACCTTACCAGTTTTGATACGTTCCAATCAATCCTCGCTCA
TAGATAACTCCAAGGGTGTTAACACTGGCCTCCACGGGGACAACATTCATGACCCATACA
GGATCATCGACTAAGGCTGAGGCGAAACCTCCAAGATGAGCGTTCATGTCGAGAAAGTTT
CTGTATCTTCCCGTCTCCGCTAGCTGTTGGTCATATTTCTTGTAGTAAGACACTCTTCTT
GCCCATTTCT
   
Sequence Name 40-K100946-0022-312-C11C-JK42
GenBank Accession LN503642 [GenBank]
Sequencing primer gene specific primer, JK42: 5'-GAGAAATGGCAGAGAAGAGTGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K100946-0022-312-C11C-JK42
ATGACACAGCTGCGGAGAGGGAAGATACAGAAACTTTCTCGACATGAACGCTCATCTTGG
AGGTTTCGCCTCAGCCTTAGTCGATGATCCTGTATGGGTCATGAATGTTGTCCCCGTGGA
GGCCAGTGTTAACACCCTTGGAGTTATCTATGAGCGAGGATTGATTGGAACGTATCAAAA
CTGGTAAGGTTACTATTCCTAATAACCGAATACACATTTTTTTGGGAAGCATTCATTGAT
TCTTTTTTATTTCTTAAACTCTTAACAGGTGTGAAGCAATGTCAACTTACCCAAGGACAT
ACGATTTCATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11