Sequences from confirmation amplicon


Confirmation sequences for line 312F08 and hit At5g06060 are shown.
Line ID 312F08
Locus At5g06060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1824820
Primers used for PCR 15fe - 8474, show primer details
   
Sequence Name 56-K111269-0022-312-F08C-15fe
GenBank Accession LN509137 [GenBank]
Sequencing primer gene specific primer, 15fe: 5'-GAACAATCTTCGAACTAATCTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K111269-0022-312-F08C-15fe
TTGTTTGGTCTAGCATTGAATGGTTTATACTTGTGGTGGTTTTGCCTTGAATGATCCGCT
ATCTGTCAATTCCATACAAGTAATCTAAGGCTTACGGTTTATCGTTGAAACATATAGTGA
TGCTTGTTGATCTTATGTAAGTTTCCACCTATTATGCAAGATCTGTTATATAACATGTTA
AACATCACCGGCACAAGTTAGGGGTATAAGTCCATTTTTTGCCCTTCTGAAAATTTCTCA
ACTTTGTTCTGTTTTCAGATAAACAATGTTGGAACTAATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCACAACGTTATT
   
Sequence Name 40-K111268-0022-312-F08C-8409
GenBank Accession LN503744 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K111268-0022-312-F08C-8409
CTGAACAGAACAAGTTGAGAATTTTCAGAAGGGCAAAAAATGGACTTATACCCCTAACTT
GTGCCGGTGATGTTTAACATGTTATATAACAGATCTTGCATAATAGGTGGAAACTTACAT
AAGATCAACAAGCATCACTATATGTTTCAACGATAAACCGTAAGCCTTAGATTACTTGTA
TGGAATTGACAGATAGCGGATCATTCAAGGCAAAACCACCACAAGTATAAACCATTTCAA
TGCTAAGACCAAACAAATATAGACACCTGATAAAAGTGTGGCAGATTAGTTCGAAGATTG
TT

Last Updated on 06.12.2018 16:11