Sequences from confirmation amplicon


Confirmation sequences for line 312H02 and hit At1g52260 are shown.
Line ID 312H02
Locus At1g52260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19461364
Primers used for PCR 21gp - 8474, show primer details
   
Sequence Name 20-K112307-0022-312-H02C-21gp
GenBank Accession LN495272 [GenBank]
Sequencing primer gene specific primer, 21gp: 5'-TTTCACAATCATCATCGCTTACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K112307-0022-312-H02C-21gp
ACGACCCGGATTCGATTCCGATCTAGACCAGCTTCTAGCAGTAGACGAGCAACTACAAGA
AGATCGACCTGAGCAACAATCAGAAGCCGAAACAGTAAGCAAAGCTCAGAGAATCGTACT
AGAGCTCAACGGTGATTACACGAAGCGAGTGATCGATGGAAACGAGTTCGTGATGGTGTT
AGGGTACGCGCCTTGGTGTGCGAGAAGTGCGGAGCTTATGCCGAGATTCGCGGAAGCTGC
GACGGCTTTGAAAGAGATTGGGAGTTCGGTTTTGATGGCTAAGATTGACGGTGATAGGTA
TTCGAAGATTGCATCGGAGCTTGAGATTAAAGGTTTCCCTACGCTTCTTCTCTTTGTTAA
TGGCACTTCTCTTACTTACAATGGTGGATCTTCTGCGTAAGTTCCCTAATTTGGCTTCTC
TAGTGATAAAGTTTTGATTTTGATTGAACCGTTTTTGAGATATGTTTTGGTCAATGTTTC
AGGGAGGATATAGTCATTTGGGTGCAAAAGAAGACTGGTGCACCAATCATTACACTTAAT
ACAGTTGATGAAAGAGTAAATGGATATCATTAATTCAATCATTACAAATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 04-K112306-0022-312-H02C-8409
GenBank Accession LN487981 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K112306-0022-312-H02C-8409
TTACATTGATTTGTAATGATTGAATTAATGATATCCATTTACTCTTTCATCAACTGTATT
AAGTGTAATGATTGGTGCACCAGTCTTCTTTTGCACCCAAATGACTATATCCTCCCTGAA
ACATTGACCAAAACATATCTCAAAAACGGTTCAATCAAAATCAAAACTTTATCACTAGAG
AAGCCAAATTAGGGAACTTACGCAGAAGATCCACCATTGTAAGTAAGAGAAGTGCCATTA
ACAAAGAGAAGAAGCGTAGGGAAACCTTTAATCTCAAGCTCCGATGCAATCTTCGAATAC
CTATCACCGTCAATCTTAGCCATCAAAACCGAACTCCCAATCTCTTTCAAAGCCGTCGCA
GCTTCCGCGAATCTCGGCATAAGCTCCGCACTTCTCGCACACCAAGGCGCGTACCCTAAC
ACCATCACGAACTCGTTTCCATCGATCACTCGCTTCGTGTAATCACCGTTGAGCTCTAGT
ACGATTCTCTGAGCTTTGCTTACTGTTTCGGCTTCTGATTGTTGCTCAGGTCGATCTTCT
TGTAGTTGCTCGTCTACTGCTAGAAGCTGGTCTAGATCGGAATCGAATCCGGGTTCGTTT
GATTCGACATTGGAATCGGGTGATGAGTAAGCGATGATGATTGTGAA

Last Updated on 06.12.2018 16:11