Sequences from confirmation amplicon


Confirmation sequences for line 313C07 and hit At1g73990 are shown.
Line ID 313C07
Locus At1g73990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27828786
Primers used for PCR KS44 - 8474, show primer details
   
Sequence Name 55-K033095-022-313-C07C-8409
GenBank Accession LN508720 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K033095-022-313-C07C-8409
CATTTACATTGAATATATCCTGAATAAAGAATATCTTTAAGCATATCAATGATGGGAGTT
GCTAAAGAATCTCGGCCAAGTTGCTGAAACATGATTCCGTCCATACGAGCTTGAACTCCC
TCGGTGATTGTTAATTCGTCGAGCAGTCCTTTTAATGTTCTGTCAACTCCAATCACCGAG
CTTCCTATACCGCTTAAGATATCTGGTAGTGATGTAGAAGGTCTTGAAAGCTCAACAAGA
GTTACCTGTATTTACCACAAGCAAAAGCCAAAAATCATATATCATCTACTTAATGCCTTC
TCAAGTTTTAAAGATTTGTTACAAAGATGAGTTCACTGAAGTAAAAGAGAGTTGAGAGGC
CAACCTTCTTATTAAGAGGAATATTAGCTTTCTGCTTAGCAATGGCTATAGCTCGGGACA
GCCCACCGACAGCATCTATTAGACCACGAGAATGAGCATCCTTACCGGTCCACACTCTGC
CCTGTGCAACTTCTTCCATCTTGTCGACCTAGGAACATGGTAAGAATAAATATTTAGTCT
TGAAAAAGCATAGGAATACAAGAAAGAAAACAAGATTGGAACAAATACAGGCATCGATCT
TGATAAGGCTGCTTTATCTCGGAAAAGCTGGTATGCATGCTGTGCA
   
Sequence Name 07-K033095-022-313-C07C-KS44
GenBank Accession LN489165 [GenBank]
Sequencing primer gene specific primer, KS44: 5'-CAGAACTGTTTGAGAAGTCTGCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K033095-022-313-C07C-KS44
GCAGCCTTATCAGATCGATGCCTGTATTTGTTCCAATCTTGTTTTCTTTCTTGTATTCCT
ATGCTTTTTCAAGACTAAATATTTATTCTTACCATGTTCCTAGGTCGACAAGATGGAAGA
AGTTGCACAGGGCAGAGTGTGGACCGGTAAGGATGCTCATTCTCGTGGTCTAATAGATGC
TGTCGGTGGGCTGTCCCGAGCTATAGCCATTGCTAAGCAGAAAGCTAATATTCCTCTTAA
TAAGAAGGTTGGCCTCTCAACTCTCTTTTACTTCAGTGAACTCATCTTTGTAACAAATCT
TTAAAACTTGAGAAGGCATTAAGTAGATGATATATGATTTTTGGCTTTTGCTTGTGGTAA
ATACAGGTAACTCTTGTTGAGCTTTCAAGACCTTCTACATCACTACCAGATATCTTAAGC
GGTATAGGAAGCTCGGTGATTGGAGTTGACAGAACATTAAAAGGACTGCTCGACGAATTA
ACAATCACCGAGGGAGTTCAAGCTCGTATGGACGGAATCATGTTTCAGCAACTTGGCCGA
GATTCTTTAGCAACTCCCATCATTGATATGCTTAAAGATATTCTTTATTCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC

Last Updated on 06.12.2018 16:11