Sequences from confirmation amplicon


Confirmation sequences for line 313G10 and hit At2g06990 are shown.
Line ID 313G10
Locus At2g06990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2897217
Primers used for PCR AH26 - 8474, show primer details
   
Sequence Name 12-K105456-0022-313-G10C-AH26
GenBank Accession LN491574 [GenBank]
Sequencing primer gene specific primer, AH26: 5'-CAGAAACCGTAAATGTTTTGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105456-0022-313-G10C-AH26
TACGCTCTTTTGTGCTAATTATGATTGTTCCTCAAATGCTGCTCTTGTAACAGTTTGCTA
TGGGTTTGAATATGCCCGCCAAAACTGTAGTGTTTACTGCTGTTAAGAAGTGGGATGGTG
ACAGCCATCGTTACATTGGGTCTGGTGAATATATCCAGGTGGGTGGTAGTTTGTTGACGT
AAGTGGTGAATTTGATGTAATATCACAAAATGATATTGTAGTAATTGTCTGTTTATATTG
AATGCTTGTAGATGAGCGGCAGGGCTGGACGTCGTGGAAAGGACGAGCGTGGCATCTGCA
TTATTATGATTGATGAACAGGTAATTTTATAAGTAATTTGATTGGAGATAATTACATCAA
TTACCGGCAACCAACTAGTAATTCCCATCCAGGAAGATCCGATATATCTCTACTGAGTAT
AGTTATATGACATAGGAATATACGGCATCCTTTATTATTTTCAAATTATATGCATTAAAG
GATAGCATCTTTTATACTGTCTTCGAAGAGGATCCTTTATTATCTCTACTGAGTATAGTT
ATATGACATAGGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGGTTATT
   
Sequence Name 28-K105457-0022-313-G10C-8409
GenBank Accession LN498845 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105457-0022-313-G10C-8409
GCCATTTACATTGAATCCTATGTCTATAACTATACTCAGTAGAGATAATAAAGGATCCTC
TTCGAAGACAGTATAAAAGATGCTATCCTTTAATGCATATAATTTGAAAATAATAAAGGA
TGCCGTATATTCCTATGTCATATAACTATACTCAGTAGAGATATATCGGATCTTCCTGGA
TGGGAATTACTAGTTGGTTGCCGGTAATTGATGTAATTATCTCCAATCAAATTACTTATA
AAATTACCTGTTCATCAATCATAATAATGCAGATGCCACGCTCGTCCTTTCCACGACGTC
CAGCCCTGCCGCTCATCTACAAGCATTCAATATAAACAGACAATTACTACAATATCATTT
TGTGATATTACATCAAATTCACCACTTACGTCAACAAACTACCACCCACCTGGATATATT
CACCAGACCCAATGTAACGATGGCTGTCACCATCCCACTTCTTAACAGCAGTAAACACTA
CAGTTTTGGCGGGCATATTCAAACCCATAGCAAACTGTTACAAGAGCAGCATTTGAGGAA
CAATCATTAATTAGCACAAAAGAGCGTTTAACAGCTGAAAAAGACAAGCTAATTCCAAAA
CATTTACGGTTTCTG
Primers used for PCR AP23 - 8474, show primer details
   
Sequence Name 27-K118837-0022-313-G10C-AP23
GenBank Accession LN498613 [GenBank]
Sequencing primer gene specific primer, AP23: 5'-AATGTATACTTACCAGCCGACCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K118837-0022-313-G10C-AP23
TTAGGCCTATTATTTCTGACTAAGTTTCTTTTCATGTTTAGCAATGTCAAATTGTAGATT
ATGGTATTCAGCAACCTCCGCCTGCGAATAATTAAAGAAGTGAGAACAGACCCAAATAAT
CTTACAGACGTCATATTATAATTTCCTGAGAGCACCTCTCCAGAAGCGTTAAGAATGGCA
GCTTCCTCTTCAAGTTTGGACACCTTATTCCCAATATCCGGTAAAGCCTGAGGTTGGAAC
ACCAGCGTAAATTAGCATAAAAAATTTGTAGATAAAATTTAGAGAGTAAAAGGACCATCC
AACAGCTACCTTTTCATGCTGGAATTGATGGAACGAGTGCCTTATAACATGCTCAGCAGT
AAATTGTCCTTCGGCACGACTCAATAGATTCAAAATTGTGTAGTAACTCAGCCTAAAAGT
ACTAAGCAAAGGGGCTGGTTTTCCCAACATCATGTCCCTGAGTGTATTCATAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 03-K118836-0022-313-G10C-8409
GenBank Accession LN487697 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118836-0022-313-G10C-8409
ATTTCATTGAATATATCCTATGAATACACTCAGGGACATGATGTTGGGAAAACCAGCCCC
TTTGCTTAGTACTTTTAGGCTGAGTTACTACACAATTTTGAATCTATTGAGTCGTGCCGA
AGGACAATTTACTGCTGAGCATGTTATAAGGCACTCGTTCCATCAATTCCAGCATGAAAA
GGTAGCTGTTGGATGGTCCTTTTACTCTCTAAATTTTATCTACAAATTTTTTATGCTAAT
TTACGCTGGTGTTCCAACCTCAGGCTTTACCGGATATTGGGAATAAGGTGTCCAAACTTG
AAGAGGAAGCTGCCATTCTTAACGCTTCTGGAGAGGTGCTCTCAGGAAATTATAATATGA
CGTCTGTAAGATTATTTGGGTCTGTTCTCACTTCTTTAATTATTCGCAGGCGGAGGTTGC
TGAATACCATAATCTACAATTTGACATTGCTAAACATGAAAAGAAACTTATGTCAGAAAT
AATTAGGCCTGAAAGGGTGCTTTGTTTTCTTGATACTGGTCGGCTGGTAAGTTATACATT

Last Updated on 06.12.2018 16:11