Sequences from confirmation amplicon


Confirmation sequences for line 314A05 and hit At5g38480 are shown.
Line ID 314A05
Locus At5g38480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15409719
Primers used for PCR BK01 - 8474, show primer details
   
Sequence Name 44-K104623-0022-314-A05C-BK01
GenBank Accession LN505176 [GenBank]
Sequencing primer gene specific primer, BK01: 5'-CACGTTCTTGTAAGCAACAGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K104623-0022-314-A05C-BK01
TCCTCACATCAACAGTTTTCGCAACTTTCTCCATGAATTCACCATTTCTTCGTAACGTTC
AGCTTGTTCGGCTAATTTCGCCATGTAAACATTCTCTTCCCTTGTCGACATCTTCGATTC
AACAATCTAGAGCAAATCCTACAAAATCAGAACCCAAAACATACACACATTGATACATTT
CTTCACCGATTAAGAACATAGAGAAAATAGGCATAAACAAATGAGTCGAAACAATAACCT
AATCATCTAGATTAAGAACACAATGAATCTTCTTCATAGGTGACGAATCTAGAGAAAATT
CTTCAACGCGAATCCGAAGTAAGATCTACATACTCGTCTATGTTCTTCACGGATAACGAA
TCTAGAGCAAAATCGTTCAAAAGCAGAAACAAAACCGAAACCTAAATTTTAACATCCTAC
ACACAAATCGGATTTAGATATCAAACACACTGAATCAGTGATAAGAACAGAGAATCGATG
AGATCGAGATCTCCAGAAACATACAATCAAAGATCAGATCTACACGTAGAAGAAGAAACT
ACGAAACGAACGATGATTAGAGAGAGAGAGATTTACCTTTCTTTTTTTTCGATTGAAGAG
AAGATCTGATTCGAATTTTGCAAGTGGTTTGAAAAAATGGAATAAGAAGAGACTGATGAA
GATACAATGTCCGGGAAACCGCGACAATATATTCAATTGATACAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 28-K104624-0022-314-A05C-8409
GenBank Accession LN498832 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104624-0022-314-A05C-8409
GAGCATTTACAATTGAATATATCCTGTATCAATTGAATATATTGTCGCGGTTTCCCGGAC
ATTGTATCTTCATCAGTCTCTTCTTATTCCATTTTTTCAAACCACTTGCAAAATTCGAAT
CAGATCTTCTCTTCAATCGAAAAAAAAGAAAGGTAAATCTCTCTCTCTCTAATCATCGTT
CGTTTCGTAGTTTCTTCTTCTACGTGTAGATCTGATCTTTGATTGTATGTTTCTGGAGAT
CTCGATCTCATCGATTCTCTGTTCTTATCACTGATTCAGTGTGTTTGATATCTAAATCCG
ATTTGTGTGTAGGATGTTAAAATTTAGGTTTCGGTTTTGTTTCTGCTTTTGAACGATTTT
GCTCTAGATTCGTTATCCGTGAAGAACATAGACGAGTATGTAGATCTTACTTCGGATTCG
CGTTGAAGAATTTTCTCTAGATTCGTCACCTATGAAGAAGATTCATTGTGTTCTTAATCT
AGATGATTAGGTTATTGTTTCGACTCATTTGTTTATGCCTATTTTCTCTATGTTCTTAAT
CGGTGAAGAAATGTATCAATGTGTGTATGTTTTGGGTTCTGATTTTGTAGGATTTGCTCT
AGATTGTTGAATCGAAGATGTCGACNAGGGAAGAGAATGTTTACATGGCGAAATTAGCCG
AACAAGCTGAACGTTACGAAGAAATGGTTGAATTCATGGAGAAAGTTGCGAAAACTGTTG
ATGTTGAGGAACTTTCAGTTGAAGAGAGGAATCTTCTCTCTGTTG

Last Updated on 06.12.2018 16:11