Sequences from confirmation amplicon


Confirmation sequences for line 315D11 and hit At5g43940 are shown.
Line ID 315D11
Locus At5g43940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17684294
Primers used for PCR AF01 - 8474, show primer details
   
Sequence Name 47-K024725-022-315-D11C-8409
GenBank Accession LN506072 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined)
>47-K024725-022-315-D11C-8409
GTTTAAACTATCTGTATTGGTAATGTATTGGTCATTGTCAATTTTCAAAGGTACCTTTAT
CCTCGATCCTCGCACTCTCACTATCTGTAGACATGTTATTGAAAAACCCTATCTCCGATT
ATTAGTTTTCTGATTTTCATTTCATTTTGACGCCGATTCACATAGCTGCGGTGGCTTACG
AGCCGAACAAGCCTCTGGTCATCGAAGATGTGCAAGTGGCTCCACCTCAAGCTGGTGAGG
TTCGGATCAAGATCCTCTACACTGCTCTTTGTCACACCGACGCTTACACTTGGAGCGGCA
AGGTTCGTTCTCTCCATTTTCATTCGTTTGAGTCTGATTTAGTGTTTTGTGGTTGATCTG
AATCGATTTATTGTTGATTAGTGAATCAATTT
   
Sequence Name 48-K024725-022-315-D11C-AF01
GenBank Accession LN506391 [GenBank]
Sequencing primer gene specific primer, AF01: 5'-CACAGCCTCAAATTGATTCACTAA-3'
Sequence (A. th genome BLAST matches underlined)
>48-K024725-022-315-D11C-AF01
AACAATAAATCGATTCAGATCAACCACAAAACACTAAATCAGACTCAAACGAATGAAAAT
GGAGAGAACGAACCTTGCCGCTCCAAGTGTAAGCGTCGGTGTGACAAAGAGCAGTGTAGA
GGATCTTGATCCGAACCTCACCAGCTTGAGGTGGAGCCACTTGCACATCTTCGATGACCA
GAGGCTTGTTCGGCTCGTAAGCCACCGCAGCTATGTGAATCGGCGTCAAAATGAAATGAA
AATCAGAAAACTAATAATCGGAGATAGGGTTTTTCAATAACATGTCTACAGATAGTGAGA
GTGCGAGGATCGAGGATAAAGGTACCTTTGAAAATTGACAATGACCAATACATTACCAAT
ACAGATAGTTTAAACAATTGTAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11