Sequences from confirmation amplicon


Confirmation sequences for line 315E09 and hit At1g31260 are shown.
Line ID 315E09
Locus At1g31260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11176522
Primers used for PCR FV25 - 8474, show primer details
   
Sequence Name 87-K033881-022-315-E09C-FV25
GenBank Accession LN514717 [GenBank]
Sequencing primer gene specific primer, FV25: 5'-CCGTACTCCGCCTACATGTATTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K033881-022-315-E09C-FV25
ATATAATATGATTTCATATCAATTCATTCAAAAGCACGACGTTTTTCAAGAAATCAAACC
ATGTGGAACTTTTTGTGTTTAAACCAAGAAAAGGAAGAAAAAAAAATCATGTGGAATTTA
GTTTATAGAACCAGAATGGATGGGAGTAATTACTCGGTCCGACTAATAAATTAAGTGGAA
AGGAGGAACTCACCTGAAGGATGCAACCGCCGAGACCCATGCCTTCGAACATTTGATGGA
AGCAAAGCGCAGCGACGAGTCCTTTGATGGTGCAAGTATTGTTAGTGTCTCCTACCGATA
GTCCTATCACTATCGACTGCACCACTATTCCTAGCTCCAATACCTATTCCATTTTATTCA
TTTCAGCTACTCACTTATTAAATCATAATTAGTGTAAATGGTTGTCACTCAAATAAAAGC
AAAAGATGAAATATAATTAGTGGAATAAATTAAAATGAAAAATTACTTGTTCCTTTTTTT
TTTAACAAAAAACTGTGTTTTATTTTTATTTTTCTAACTAACACAACAAACAGGGTAACA
ATTTTGTTTTACACTATGCAACCTACAATTAAAATTTGAGTTACCAGTTAACCTTAAAAA
AATGGCATATTAATGCCTTCGTATGTGATAATGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTA
   
Sequence Name 31-K033881-022-315-E09C-8409
GenBank Accession LN500062 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K033881-022-315-E09C-8409
GAAGCCATTTACATTATCACATACGAAGGCATTAATATGCCATTTTTCTAAGGTTAACTG
GTAACTCAAATTTTAATTGTAGGTTGCATAGTGTAAAACAAAATTGTTACCCTGTTTGTT
GTGTTAGTTAGAAAAATAAAAATAAAACACAGTTCTTTGTTAAAAAAAAAAGGAACAAGT
AATTTTTCATTTTAATTTATTCCACTAATTATATTTCATCTTTTGCTTTTATTTGAGTGA
CAACCATTTACACTAATTATGATTTAATAAGTGAGTAGCTGAAATGAATAAAATGGAATA
GGTATTGGAGCTAGGAATAGTGGTGCAGTCGATAGTGATAGGACTATCGGTAGGAGACAC
TAACAATACTTGCACCATCAAAGGACTCGTCGCTGCGCTTTGCTTCCATCAAATGTTCGA
AGGCATGGGTCTCGGCGGTTGCATCCTTCAGGTGAGTTCCTCCTTTCCACTTAATTTATT
AGTCGGACCGAGTAATTACTCCCATCCATTCTGGTTCTATAAACTAAATTCCACATGATT
TTTTTTTCTTCCTTTTCTTGGTTTAAACACAAAAAGTTCCACATGGTTTGATTTCTTGAA
AAACGTCGTGCTTTTGAATGAATTGATATGAAATCATATATATGATGAGAACTTGATAAT
ACATGTA

Last Updated on 06.12.2018 16:11