Sequences from confirmation amplicon


Confirmation sequences for line 316H09 and hit At3g13830 are shown.
Line ID 316H09
Locus At3g13830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4554739
Primers used for PCR 52sf - 8474, show primer details
   
Sequence Name 45-K116453-0022-316-H09C-52sf
GenBank Accession LN505651 [GenBank]
Sequencing primer gene specific primer, 52sf: 5'-TGCTTGTTGGAGGGATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K116453-0022-316-H09C-52sf
AGCAAACCGTAACCACAAAATCTTTAGAAGAAACTTGTTTCTGGTGGTTATGAAATCTAC
AGTTTCAAATCCGATTCATGGAAAGATCTTAATGTTGATCTCGGGGAATCTACAGAACTT
TGTGAACTAGGAAGCGTGTCTTTGAAGGGAAATGCTTACTTTTCGGTTATAACGAAACAT
GAAGAAGAAGAACTTTGGGAAAATAATTTACTCTGTTTTGACTTCACGACGGAGAGTTTT
GGAAAGTTTCTGTCTCTGCCGTTTGACAATGTGACTGCTGAAGAAGAATTCGGCATTATG
GTTTTATCTTGTGTTAGAGATGAGCATCTTGCTGTGTTATATCAACTTGACACTTTAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACGATTTATTATA
   
Sequence Name 12-K116452-0022-316-H09C-8409
GenBank Accession LN491743 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116452-0022-316-H09C-8409
AGCAGATGCTCATCTCTACACAAGATAAAACCATAATGCCGAATTCTTCTTCAGCAGTCA
CATTGTCAAACGGCAGAGACAGAAACTTTCCAAAACTCTCCGTCGTGAAGTCAAAACAGA
GTAAATTATTTTCCCAAAGTTCTTCTTCTTCATGTTTCGTTATAACCGAAAAGTAAGCAT
TTCCCTTCAAAGACACGCTTCCTAGTTCACAAAGTTCTGTAGATTCCCCGAGATCAACAT
TAAGATCTTTCCATGAATCGGATTTGAAACTGTAGATTTCATAACCACCAGAAACAAGTT
TCTTTCTAAAGATTTTGTGGTTACGGTTTTTGCTGCTATCATATCCGAAAGCATACGAGC
CGAAGTTGTCGAATCCTCCCAACAAGCAA

Last Updated on 06.12.2018 16:11