Sequences from confirmation amplicon


Confirmation sequences for line 317A09 and hit At2g37170 are shown.
Line ID 317A09
Locus At2g37170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15613710
Primers used for PCR PC62 - 8474, show primer details
   
Sequence Name 68-K103791-0022-317-A09C-PC62
GenBank Accession LN512001 [GenBank]
Sequencing primer gene specific primer, PC62: 5'-AACTCTCTAGCAGACGGCTACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K103791-0022-317-A09C-PC62
TTGGACATTCGTTCTCGTCTACACAGTCTTCTCCGCTACTGATCCCAAACGTAACGCTAG
AGACTCCCACGTTCCGGTTAGTATTTATTTCTATCAGTTATAAGATCGTTCTGGTAACAT
ACGATAACATTAATTAATACTTAGTGTTATGAATTATGAAACGAAAGGTTTTGGCGCCAC
TTCCGATTGGGTTTGCGGTGTTTATGGTACATTTGGCCACTATTCCGATCACCGGAACCG
GCATCAACCCGGCTAGGAGTTTCGGAGCTGCCGTAATCTATAACAAGAGCAAGCCATGGG
ATGACCACGTACGTTTCTATTTCTTAATTATTTAAAATTAATCAAATTATGGACTATTGT
TTGATGGGAAAATAATGAACTGTTTTTTTGGTCTATCTAAATACAGTGGATATTCTGGGT
GGGACCATTCATTGGAGCTGCGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTC
   
Sequence Name 84-K103792-0022-317-A09C-8409
GenBank Accession LN514422 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K103792-0022-317-A09C-8409
GCCATTTCCAGCTCCATGAATGGTCCCACCCAGAATATCCACTGTATTTAGATAGACCAA
AAAAACAGTTCATTATTTTCCCATCAAACAATAGTCCATAATTTGATTAATTTTAAATAA
TTAAGAAATAGAAACGTACGTGGTCATCCCATGGCTTGCTCTTGTTATAGATTACGGCAG
CTCCGAAACTCCTAGCCGGGTTGATGCCGGTTCCGGTGATCGGAATAGTGGCCAAATGTA
CCATAAACACCGCAAACCCAATCGGAAGTGGCGCCAAAACCTTTCGTTTCATAATTCATA
ACACTAAGTATTAATTAATGTTATCGTATGTTACCAGAACGATCTTATAACTGATAGAAA
TAAATACTAACCGGAACGTGGGAGTCTCTAGCGTTACGTTTGGGATCAGTAGCGGAGAAG
ACTGTGTAGACGAGAACGAATGTTCCAATGATCTCTGCGGCTAGTCCGGTGCCTGTGTTG
TAGCCGTCTGCTAGAGAGT

Last Updated on 06.12.2018 16:11