Sequences from confirmation amplicon


Confirmation sequences for line 317B08 and hit At2g22910 are shown.
Line ID 317B08
Locus At2g22910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9750712
Primers used for PCR BV22 - 8474, show primer details
   
Sequence Name 42-K105952-0022-317-B08C-BV22
GenBank Accession LN504470 [GenBank]
Sequencing primer gene specific primer, BV22: 5'-GTACCTTCGTCGTTACTATCTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K105952-0022-317-B08C-BV22
CGTTTAAGGCACATACTTATTCTTTATCGTTCAGTGTTTGGTTTATGCACGCAAGGTGTT
TGATTGTTTGTCTTGCTTTAAACTTGAACATGTAATTTGTATTTCTATAGTGCGTATCGT
CTTATTGATTCATCTGCAACTATGTAGCTTTGAGTGTGCGAGAGGAAGCTAGTTTATGAC
CAGTGTGCTTTAGATTTACATTCTTGTTCAGAGGATTCCAAAGTGAAATCATTATAACAT
GTGTGCAGGACATAGCATTTCTTCATCATCTGGGCATTAAGTTTGTTCTAGTTCCTGGAA
CTCAAGTGCAAATCGACCAGCTTCTAGCTGAGAGAGGTCAGAAAAAATTAACCTTGCCAC
TTGTCTTCTTATTGGCTTGTTTAGATATTTGTCGGCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 58-K105953-0022-317-B08C-8409
GenBank Accession LN509710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K105953-0022-317-B08C-8409
GCCATTTACATTGAATATATCCTGCCGACAATATCTAAACAAGCCAATAAGAAGACAAGT
GGCAAGGTTAATTTTTTCTGACCTCTCTCAGCTAGAAGCTGGTCGATTTGCACTTGAGTT
CCAGGAACTAGAACAAACTTAATGCCCAGATGATGAAGAAATGCTATGTCCTGCACACAT
GTTATAATGATTTCACTTTGGAATCCTCTGAACAAGAATGTAAATCTAAAGCACACTGGT
CATAAACTAGCTTCCTCTCGCACACTCAAAGCTACATAGTTGCAGATGAATCAATAAGAC
GATACGCACTATAGAAATACAAATTACATGTTCAAGTTTAAAGCAAGACAAACAATCAAA
CACCTTGCGTGCATAAACCAAACACTGAACGATAAAGAATAAGTATGTGCCTTTAAAACG
AGATCACAATAAGGGCCGTCTAAGACATCGCCGGAGATA

Last Updated on 06.12.2018 16:11