Sequences from confirmation amplicon


Confirmation sequences for line 317E12 and hit At5g63600 are shown.
Line ID 317E12
Locus At5g63600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25461698
Primers used for PCR MA02 - 8474, show primer details
   
Sequence Name 37-K017280-022-317-E12C-MA02
GenBank Accession LN502432 [GenBank]
Sequencing primer gene specific primer, MA02: 5'-TGATCTAAGCGTTTCTGATGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K017280-022-317-E12C-MA02
TGAAAGCGAGTGAAGAGTGGGGAGTTTTTCAGGTGGTTAACCATGGGATTCCAACCGAGC
TGATGCGGCAGCTGCAAATGGTTGGTACGCAGTTTTTCGAGCTCCCGGATGCTGAAAAGG
AAACCGTGGCGAAAGAGGAAGACTTCGAAGGATACAAGAAGAATTATCTAGGAGGTATAA
ACAATTGGGACGAACATCTCTTTCACAGACTCTCTCCACCCTCAATTATCAATTATAAAT
ATTGGCCTAAGAATCCTCCTCAATATAGGTACGTACCTTTCCTAATAAAATCGTATGAGT
TAGTAATTCATGTTTTACGTTAAATTTCATTTCTTTAACAAAATATCAAATTTTAAACGT
TGGATTTTCGTCCAAATTTTTGGGGGTACCAGGGAGGTGACGGAGGAGTATACAAAGCCA
TATATTCAGCAATTGTATACAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCT
   
Sequence Name 38-K017280-022-317-E12C-8409
GenBank Accession LN502811 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K017280-022-317-E12C-8409
AGATTTCTCGGACATGAAGCCATTTACAATTGAATATATTGTATACAATTGCTGAATATA
TGGCTTTGTATACTCCTCCGTCACCTCCCTGGTACCCCCAAAAATTTGGACGAAAATCCA
ACGTTTAAAATTTGATATTTTGTTAAAGAAATGAAATTTAACGTAAAACATGAATTACTA
ACTCATACGATTTTATTAGGAAAGGTACGTACCTATATTGAGGAGGATTCTTAGGCCAAT
ATTTATAATTGATAATTGAGGGTGGAGAGAGTCTGTGAAAGAGATGTTCGTCCCAATTGT
TTATACCTCCTAGATAATTCTTCTTGTATCCTTCGAAGTCTTCCTCTTTCGCCACGGTTT
CCTTTTCAGCATCCGGGAGCTCGAAAAACTGCGTACCAACCATTTGCAGCTGCCGCATCA
GCTCGGTTGGAATCCCATGGTTAACCACCTGAAAAACTCCCCACTCTTCACTCGCTTTCA
CCACCTCACGCACCAAGAAGTCTTCATCAGAAACGCTTAGATC

Last Updated on 06.12.2018 16:11