Sequences from confirmation amplicon


Confirmation sequences for line 318F07 and hit At2g37770 are shown.
Line ID 318F07
Locus At2g37770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15836378
Primers used for PCR GC82 - 8474, show primer details
   
Sequence Name 54-K033708-022-318-F07C-GC82
GenBank Accession LN508390 [GenBank]
Sequencing primer gene specific primer, GC82: 5'-TATGCAAACAACACATTTTCGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K033708-022-318-F07C-GC82
GCATTGTAAAATAGATCCTTTTTCTATAAAAAAAAACTTTGTAAATAAATAACATCCATA
AGAAACTGACAAGCACAATACAGTTTCATGTTCTACTCAAAGTTGCATATAATGTAAAGA
AGGTTTTTTCTGGTTGAATAAATTTAACATTCATTAAAAAAAAAAAAATACTGACTAGTT
CATCAAAGAGAGAAACCTAGCTCCCATTATTTAAATCAGCTAAAAATCATATCTCGCCAT
CCCATAATTCTTCAATAAACTTGTAAGGGCTTAGTGTCTCATGAACAAGGAAGGAACCAG
TGACTAACCTAGCCTGCATTTAATCCAAATAAATTACTATCAATACACGATCAAAACAAG
GAAAAGCAAAGGCAAATCAACTAATTTTATGCAATGAAATGTTAAAAACGTACCTGTTCA
ATCTCAGCAAACTTAGCGAACATGTAATCGGGTATTGACCAGTCAAAAACATTAAAGTTC
TCTTTGATCCTTCCTTCATTTGTACTCTTGGGAAGCACACTGGAAATCTACATGGATCAC
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAAATGTCCCCACCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCC
   
Sequence Name 46-K033708-022-318-F07C-8409
GenBank Accession LN505782 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K033708-022-318-F07C-8409
CAGAGTACAAATGAAGGAAGGATCAAAGAGAACTTTAATGTTTTCGACTGGTCAATACCC
GATTACATGTTCGCTAAGTTTGCTGAGATTGAACAGGTACGTTCTTAACATTTCATTGCA
TAAACTTAGTTGATCTGCCTCTGCTCTTCCTCGTCTTGATCGTGTATTGATAGTAATTTA
TTTGGATTAAATGCAGGCTAGGTTAGTCACTGGTTCCTTCCTTGTTCATGAGACACTAAG
CCCTTACAAGTCTATTGAAGAATTATGGGATGGCGAGATATGATTTTTAGCTGATCTAAA
TAATGGGAGCTAGGTTTCTCTCTCTGATGAACTAGTCAGTATCTTTTTTTTTTTAATGAA
TGTTAAATTTATTCAACCAGAAAAAACCTTCTTTACATTATATGCAACTTTGAGTAGAAC
ATGAAACTGTATTGTGCTTGTCAGTTTCTTATGGATGTTATTTATTTACAAAGTTTTTTT
TTATAGAAAAAGGATCTATTTATACAATTGCAATGTATTATAGTCATTTCAAATCGAAAA
TG

Last Updated on 06.12.2018 16:11