Sequences from confirmation amplicon


Confirmation sequences for line 319F10 and hit At4g30530 are shown.
Line ID 319F10
Locus At4g30530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14921292
Primers used for PCR TH15 - 8474, show primer details
   
Sequence Name 09-K103674-0022-319-F10C-TH15
GenBank Accession LN490143 [GenBank]
Sequencing primer gene specific primer, TH15: 5'-GATCGCTATGCTATCAGGAATCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K103674-0022-319-F10C-TH15
CGTATCGCATCCTTGACGATGGTTATGTCTCCAGTTTAAGTTCTGGTCCCTTCTTTGCTC
TTCCGACTGTTCCTCCCCTTACCCTGGCTATGATCTAAAGGAAAAATCGTTTTCAATTAG
AAGAATATTTTTTAATCAACAACATGCACTATTCAGTTTTTGAATCATTCCAAATGATAT
CACAACACTAATTCAAACCAATAAAAAAAATAAGATGAATACTCGCCACTAGTGAAGAAT
CAGTCCCTTACTCTTATAGATAATAATCTTTATTGTCATGCTTTATTGAATACTCTGCAC
TACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGC
   
Sequence Name 25-K103675-0022-319-F10C-8409
GenBank Accession LN497406 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K103675-0022-319-F10C-8409
GCCATTTACAATTGAATATATCCTGTAGTGCAGAGTATTCAATAAAGCATGACAATAAAG
ATTATTATCTATAAGAGTAAGGGACTGATTCTTCACTAGTGGCGAGTATTCATCTTATTT
TTTTTATTGGTTTGAATTAGTGTTGTGATATCATTTGGAATGATTCAAAAACTGAATAGT
GCATGTTGTTGATTAAAAAATATTCTTCTAATTGAAAACGATTTTTCCTTTAGATCATAG
CCAGGGTAAGGGGAGGAACAGTCGGAAGAGCAAAGAAGGGACCAGAACTTAAACTTGGAG
ACATAACCATCGTCAAGGATGCGATTACGCCTGGAAGTTACTTCGGAAACGAGATTCCTG
ATAGCATAGCGA

Last Updated on 06.12.2018 16:11