Sequences from confirmation amplicon


Confirmation sequences for line 319H09 and hit At1g34110 are shown.
Line ID 319H09
Locus At1g34110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12418562
Primers used for PCR EQ18 - 8474, show primer details
   
Sequence Name 39-K102871-0022-319-H09C-EQ18
GenBank Accession LN503314 [GenBank]
Sequencing primer gene specific primer, EQ18: 5'-CATCGTAGCAGTGAAGAAACTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K102871-0022-319-H09C-EQ18
ATCAACATAGATTCATTCGCTGCAGAGATTCAGATTCTCGGTAACCTCAGACACAGAAAC
ATAGTGAAGCTATTAGGTTACTGTCCGAACAAATCAGTGAAGCTGCTTCTATACAATTAC
TTCCCTAACGGGAATCTACAACAGTTGTTGCAAGGAAATCGAAACTTGGATTGGGAAACA
CGTTACAAGATTGCGATTGGAGCTGCTCAGGGTTTGGCTTATCTTCATCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TACNTTCACGTCCAAATGGG
   
Sequence Name 39-K102876-0022-319-H09C-8409
GenBank Accession LN503315 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K102876-0022-319-H09C-8409
TCGGATGACCATTTACAATTGAATATATCCTGATGAAGATAAGCCAAACCCTGACCAGCT
CCAATCGCAATCTTGTAACGTGTTTCCCAATCCAAGTTTCGATTTCCTTGCAACAACTGT
TGTAGATTCCCGTTAGGGAAGTAATTGTATAGAAGCAGCTTCACTGATTTGTTCGAACAG
TAACCTAATAGCTTCACTATGTTTCTGTGTCTGATGTTACCGAGAATCTGAATCTCTGCA
GCGAATGAATCTATTGTTGATTCTCCTTCTTCGTTGTTGTCTTTAGTTTTCCAGAGTTTC
TTCACTGCTACGATG
Primers used for PCR KH53 - 8474, show primer details
   
Sequence Name 77-K118656-0022-319-H09C-KH53
GenBank Accession LN513612 [GenBank]
Sequencing primer gene specific primer, KH53: 5'-AAAACAATCTTTTGCCAATAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K118656-0022-319-H09C-KH53
AATTCTTATTAAAAAAATCCTAATATTTTGTAATGATGCACAAGAAACCCTTTTGTTTTT
TTAACAAATTCATTCAAAATTTCTCTCAATCTCTTGTAATAATGGATATGAAGCTCTGAA
TTTTAATAAATACACTAAAACCCCCTATCTCTTATAATAATGCAAATGAAACCTTGAGCT
TTATCAAATTTATCAAAAAGGCCCTCCCGTTAAAAAAGAGAGAAACTTGCCTGGTGCGAT
GTATCCATAAGAACCAGCAACACGAGACATGGCGTTATGATAATTCGGCGAATTCATCAT
CAGCTTCGCAAGTCCAAAATCAGCAAGAATCGCTTCATATTTCGAATCCAGAAGAATATT
ATTACACTTGACATCTCTATGCAAAATCTACATGGCTTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 51-K118655-0022-319-H09C-8409
GenBank Accession LN507646 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118655-0022-319-H09C-8409
TTGAAGCCATGTAGATTTTGCATAGAGATGTCAAGTGTAATAATATTCTTCTGGATTCGA
AATATGAAGCGATTCTTGCTGATTTTGGACTTGCGAAGCTGATGATGAATTCGCCGAATT
ATCATAACGCCATGTCTCGTGTTGCTGGTTCTTATGGATACATCGCACCAGGCAAGTTTC
TCTCTTTTTTAACGGGAGGGCCTTTTTGATAAATTTGATAAAGCTCAAGGTTTCATTTGC
ATTATTATAAGAGATAGGGGGTTTTAGTGTATTTATTAAAATTCAGAGCTTCATATCCAT
TATTACAAGAGATTGAGAGAAATTTTGAATGAATTTGTTAAAAAAACAAAAGGGTTTCTT
GTGCATCATTACAAAATATTAGGATTTTTTTTTAATAATGAATTTGTTAAAATTTGAGGT
TTCATTTGCATTATTGGCAAAGAATTGTTTT

Last Updated on 06.12.2018 16:11