Sequences from confirmation amplicon


Confirmation sequences for line 320E02 and hit At3g11402 are shown.
Line ID 320E02
Locus At3g11402 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3575499
Primers used for PCR JC12 - 8474, show primer details
   
Sequence Name 93-K101673-0022-320-E02C-8409
GenBank Accession LN515243 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K101673-0022-320-E02C-8409
ATTTACATTGAATATATACTGTTGCTTAAAACTTCCATTTTTGTGAAGTAATGATATAAT
GTGAAACCAGGCTTCACATAACCAGAGGATCCGAATACACATTGAAGATGGACAGTGATA
CAACATTTGTTGCATGCGTAAAACCAATCCCTTGGATCTACCTCTTTCTCACATACTTCA
CACCAATACGTACCAAACACTACTTCTCCGTAGCAAAGGGAGAGAAAATGCTTGTCGTAT
TTGTAGTAGACTTCGTTTGGAATGGTAGCGCACTGATAACACATAGCAAATTTGCATTCA
GAACACTGTAGGTAGGACACAAGACATATCTCCTTGCAAGCCTCGCAACAAATCTAACTT
TTCCCTTTCTCGGAAGTGGATATTNAATAGGG
   
Sequence Name 93-K101680-0022-320-E02C-JC12
GenBank Accession LN515244 [GenBank]
Sequencing primer gene specific primer, JC12: 5'-CCCTATTTATATCCACATCCGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K101680-0022-320-E02C-JC12
CAGGAGATATGTCTTGTGTCCTACCTACAGTGTTCTGAATGCAAATTTGCTATGTGTTAT
CAGTGCGCTACCATTCCAAACGAAGTCTACTACAAATACGACAAGCATTTTCTCTCCCTT
TGCTACGGAGAAGTAGTGTTTGGTACGTATTGGTGTGAAGTATGTGAGAAAGAGGTAGAT
CCAAGGGATTGGTTTTACGCATGCAACAAATGTTGTATCACTGTCCATCTTCAATGTGTA
TTCGGATCCTCTGGTTATGTGAAGCCTGGTTTCACATTATATCATTACTTCACAAAAATG
GAAGTTTTAAGCAACAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11