Sequences from confirmation amplicon


Confirmation sequences for line 320H07 and hit At2g18130 are shown.
Line ID 320H07
Locus At2g18130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7882065
Primers used for PCR BV25 - 8474, show primer details
   
Sequence Name 08-K105747-0022-320-H07C-BV25
GenBank Accession LN489732 [GenBank]
Sequencing primer gene specific primer, BV25: 5'-TTAGAACCATCCTCATTTAATGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K105747-0022-320-H07C-BV25
CTCGACCGCATTATCTCCTTGTGTTATGTGAACCTGAGAAAAGAGCAAAGGTAAAAGCTC
ATAAGTCCTAAGTGACGAAAGAAATTTACATATACATTCTTATAACACTTTTATAAAATA
AATCCTAGAATTTAAAGTTATTTACGAGTACATACCATTAATATTAATTGTTTAGATATA
TTTTATTTAAGTAAAGATGTAAATCAAATTCTATATTCATTTAGAAATGGTCATTTAGAA
ATCTAACATTATTAATTAATTTCAAATAAACCATTTTGAAATTGTGGTTTGAAAAGATTG
TAACAAATATATTTTGTTTCTATATTCTATATTCTAAATTCTATGTACAATGATATCAGA
ATGAAAACTTATACTTATTACTAGTAAAATTAATAATAAAAAAGCAAAGAAAATCAAATC
GAATTATAATCTATATTTCCTTCAAGGCGATAATTGGATTTATTTTTTATGAAACTTTAT
GAACATTTATTATTCTTCAAACGGATGACTAAGGATTTTTCTAAAATTTGAACTTACATG
TAAATGGCGGATATTTGTCAGGATATATTCAATTGAATATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 24-K105748-0022-320-H07C-8409
GenBank Accession LN496992 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K105748-0022-320-H07C-8409
ATTCAATTGAATATATCCTGACAATATCCGCCATTTACATGTAAGTTCAAATTTTAGAAA
AATCCTTAGTCATCCGTTTGAAGAATAATAAATGTTCATAAAGTTTCATAAAAAATAAAT
CCAATTATCGCCTTGAAGGAAATATAGATTATAATTCGATTTGATTTTCTTTGCTTTTTT
ATTATTAATTTTACTAGTAATAAGTATAAGTTTTCATTCTGATATCATTGTACATAGAAT
TTAGAATATAGAATATAGAAACAAAATATATTTGTTACAATCTTTTCAAACCACAATTTC
AAAATGGTTTATTTGAAATTAATTAATAATGTTAGATTTCTAAATGACCATTTCTAAATG
AATATAGAATTTGATTTACATCTTTACTTAAATAAAATATATCTAAACAATTAATATTAA
TGGTATGTACTCGTAAATAACTTTAAATTCTAGGATTTATTTTATAAAAGTGTTATAAGA
ATGTATATGTAAATTTCTTTCGTCACTTAGGACTTATGAGCTTTTACCTTTGCTCTTTTC
TCAGGTTCACATAACACAAGGAGATAATGCGGGTCGAGCTATGATCATTTCGTGGGTAAT
GCCATTAAATGAGGAGGGTTCTAAAA

Last Updated on 06.12.2018 16:11