Sequences from confirmation amplicon


Confirmation sequences for line 321D05 and hit At4g15960 are shown.
Line ID 321D05
Locus At4g15960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9046909
Primers used for PCR NB58 - 8474, show primer details
   
Sequence Name 05-K034547-022-321-D05C-8409
GenBank Accession LN488254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K034547-022-321-D05C-8409
TGAGCCATTTACATTGAATATATCCTGTGTCGCCACGTGTACCAAAGCTCCGGAAATCCG
TGGAGGAAGAGTATGATCGGATCTTCTCCGGATCCGGATCCGGGTTTCTCCGCCACGTGC
ATGTTTATGCCATTTACCTTTAGGGTTTTGTGTTCTACGCCGTCGAGTCTCGACTTCTCT
GGTCGTTTTGTTTGATTGTCGGGTAATGTAGAGAAATGGGTAGTTGGATTTGGGTTTTCC
CCAAGAAAACGACGGAGTCTGAGAGGAAAATGGTGTCGGAATCGTGAAAGTGAGAGTTTT
CTACACAAGAAGAACATGTGTAGTTTGATGTGTTTGGCTGCGAGAAGACGTTGTGTGGAT
AAATCTCCGGTCGTTTAAGAATACCGAACCAAT
   
Sequence Name 06-K034547-022-321-D05C-NB58
GenBank Accession LN488706 [GenBank]
Sequencing primer gene specific primer, NB58: 5'-GACCATTGGTTCGGTATTCTTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K034547-022-321-D05C-NB58
ACACGTCTTCTCGCAGCCAACACATCAAACTACACATGTTCTTCTTGTGTAGAAAACTCT
CACTTTCACGATTCCGACACCATTTTCCTCTCAGACTCCGTCGTTTTCTTGGGGAAAACC
CAAATCCAACTACCCATTTCTCTACATTACCCGACAATCAAACAAAACGACCAGAGAAGT
CGAGACTCGACGGCGTAGAACACAAAACCCTAAAGGTAAATGGCATAAACATGCACGTGG
CGGAGAAACCCGGATCCGGATCCGGAGAAGATCCGATCATACTCTTCCTCCACGGATTTC
CGGAGCTTTGGTACACGTGGCGACACAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGCCCAAATGGGAGA
ACATGAA

Last Updated on 06.12.2018 16:11