Sequences from confirmation amplicon


Confirmation sequences for line 322B03 and hit At3g16895 are shown.
Line ID 322B03
Locus At3g16895 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5771480
Primers used for PCR 18we - 8474, show primer details
   
Sequence Name 64-K118393-0022-322-B03C-18we
GenBank Accession LN511294 [GenBank]
Sequencing primer gene specific primer, 18we: 5'-ACATTTCACTGGACATGGAATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118393-0022-322-B03C-18we
GCTCTAAGACAGTCCTTATTACATTCATCATTCCAGTAATCTTCGGAACACCTAATATTG
CAGTTGTCAAAAGAGAAGCCTTCGATCCTTGCATCTACACAAGTCCACATATATATCAAA
CATCATCAATACCAACATGTGTAAATTTTATATCATTCCAATAAAAAATAGTTATATAAC
ATGTCCAAATAAAAGTTTTTAACGAAACTTTTGAATTCATGTATAATATATATATAAACA
AAAACGTGATCATAACGAATTCCAAATATTTAGATATATAGCCTTTGAAATGATCACTAC
TAAATTGCAAAATAGCTAAACCAAACTTTGAAAATACCTGAAGCCAAAACGTTGTTGTTA
GGCAACGAAACTGCAAGTATTATTACCAATTTACATGGATCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 16-K118392-0022-322-B03C-8409
GenBank Accession LN493628 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118392-0022-322-B03C-8409
TTGATCCATGTAAATTGGTAATAATACTTGCAGTTTCGTTGCCTAACAACAACGTTTTGG
CTTCAGGTATTTTCAAAGTTTGGTTTAGCTATTTTGCAATTTAGTAGTGATCATTTCAAA
GGCTATATATCTAAATATTTGGAATTCGTTATGATCACGTTTTTGTTTATATATATATTA
TACATGAATTCAAAAGTTTCGTTAAAAACTTTTATTTGGACATGTTATATAACTATTTTT
TATTGGAATGATATAAAATTTACACATGTTGGTATTGATGATGTTTGATATATATGTGGA
CTTGTGTAGATGCAAGGATCGAAGGCTTCTCTTTTGACAACTGCAATATTAGGTGTTCCG
AAGATTACTGGAATGATGAATGTAATAAGGACTGTCTTAGAGCTGGCTTTCAAAAAGGTG
GACAGTGTGGTTCTCCTTGTATTCCATGCCGGGGGAAATGT

Last Updated on 06.12.2018 16:11