Sequences from confirmation amplicon


Confirmation sequences for line 322C03 and hit At3g05380 are shown.
Line ID 322C03
Locus At3g05380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1544170
Primers used for PCR TM25 - 8474, show primer details
   
Sequence Name 54-K103693-0022-322-C03C-TM25
GenBank Accession LN508452 [GenBank]
Sequencing primer gene specific primer, TM25: 5'-CTGTCACAAAAGGAGATGATCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103693-0022-322-C03C-TM25
TCATTGTGTAATGGAACTGGTATTGTATTTGAACTACCATAACCAACTCAACGCCCAAGT
CATCGAAGAGAACATTGCATTTGTTATGATCAACAGTGAGAATTTTTCCATCATGAATCT
CCCGTGTTTTGGGATGGATGGCAATGACTCTGTTCCCAACTGCTAATGGCCGAGCCAAAT
CTGTAGGAAGCCCCTCCCTAGCACCCGTTCGAAGCTCTGTATAATGCTTTCGCACAGATT
CGCGGTACTGTTTAAGTTTCTCCCGCTCTTCATGTAAAAATCTCTCAGAGAATCTTCGTG
GTCTACCAAGAGAACTGCAAAAAAACGAAAGAAACCTTTTATAATCTACCTATATTCTCT
GGAAGTATCCAATAACATAACTTGAACAAACCTTTTAATGACGCTCCATTCAAGACGAGT
AAGTCTTGGAATGTGACCAAGCCCCACGTGATTTACAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 70-K103694-0022-322-C03C-8409
GenBank Accession LN512375 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K103694-0022-322-C03C-8409
GCCTTTACAATTGAATATATCCTGTAAATCACGTGGGGCTTGGTCACATTCCAAGACTTA
CTCGTCTTGAATGGAGCGTCATTAAAAGGTTTGTTCAAGTTATGTTATTGGATACTTCCA
GAGAATATAGGTAGATTATAAAAGGTTTCTTTCGTTTTTTTGCAGTTCTCTTGGTAGACC
ACGAAGATTCTCTGAGAGATTTTTACATGAAGAGCGGGAGAAACTTAAACAGTACCGCGA
ATCTGTGCGAAAGCATTATACAGAGCTTCGAACGGGTGCTAGGGAGGGGCTTCCTACAGA
TTTGGCTCGGCCATTAGCAGTTGGGAACAGAGTCATTGCCATCCATCCCAAAACACGGGA
GATTCATGATGGAAAAATTCTCACTGTTGATCATAACAAATGCAATGTTCTCTTCGATGA
CTTGGGCGTTGAGTTGGTTATGGTAGTTCAAATACAATACCAGTTCCATATACACAATGA
TTTATTGGTAAAATAAGGTCTCAGTTGTTTGATCATCTCCTT
Primers used for PCR TM26 - 8474, show primer details
   
Sequence Name 55-K103693-0022-322-C03C-TM26
GenBank Accession LN508786 [GenBank]
Sequencing primer gene specific primer, TM26: 5'-CTCCGTGATCTCTGTTTTCTATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103693-0022-322-C03C-TM26
TGCTATTGAGTATTCAAATTTGTGATTCTGAAGAAACATTACCTCATATGATTTGGTTTT
TTATGTTGCAGGAATTGCCTCAAGATGAGAATAATATGAAGTCTTTGGTTAAAACAAAAC
GCGCTGGTCAAGTTCCGGCTCAGTCAAAACAGATGAAAACTGTTAAGGCGTTGGAGGAAT
CTGCTATAACAAGTGATAAGAAAAGACCTGGGATGGATATAGTAGCGTCACCTAAACAAG
TTTCTGACTCGGGTCCAACCAGTTTATCGCAGAAACCTCCAAACAGGCGAAAGAAGAGTC
TGCAGAAAAGCTTACAAGAAAAGGCTAAATCTTCTGAAACCACTCATAAAGCTGCACGTA
GTTCCAGATCTCTTTCAGAACAAGAGTTGTTGTTAAAGGTGGAGCCTCTAACTTCACTTC
TCCTACGCACATCAGTATCTTGTACATCTATATATTTGTTTATTCCAATTTCAGGATAAG
CTTGCTACTTCTCTGTCGTTTCCCTTTGCACGTCGAAGGTGCATATTTGAATGGTTTTAT
AGTGCTATCGACCATCCCTGGTTTTCAAAGATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT

Last Updated on 06.12.2018 16:11