Sequences from confirmation amplicon


Confirmation sequences for line 322C04 and hit At5g47175 are shown.
Line ID 322C04
Locus At5g47175 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19160234
Primers used for PCR 05xq - 8474, show primer details
   
Sequence Name 36-K119542-0022-322-C04C-05xq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 05xq: 5'-ATATGTTATGGGCTTTAACGGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K119542-0022-322-C04C-05xq
GCATTTGTTTGATATTGGACCTTTTTGGGCTTTTAGTTGGATCTTTTGGACCCAATTCAG
ATTTGTTTTATAATTTGGGCATTCACTTCATTCACTTAATGATATTGATTATTTTTTTGT
TAAGTACAGATTTATCATTTTTTTTTCTCAGATTTATCATTTTTAATGACTCTTGAAATC
TTGTACCCAAATTTTTTTAATAGAATCTTAAAATACATGACCTTATATCAGTATTAGATT
TAAATTTTCTTTGAAAAGTTGAGAAACCAATGACGGTATTAATCTATTTTGAAAACACAT
GGATTTTTTTCTATATATTTATATATATAATTCATTGACGGTATTATATATATATATATA
TATGGTAATTTGATTCATTCATTCATTTGAAGTATGATAAAAAAAAGTTGATACACCCAA
ATTACACTAATTAATATCATCAATACACAACGGTTATGATAAATAAATATAGAAAAGATT
TTCTCTTTCAAAAACGAGAACCATATTCACTATTATTATTTTTCTTCAAAATAAATCTCA
TTAGAGATTGTCATTTTTGCCTATCATATTCATATATTGGATGAAACTCCAAAGTTATCT
AATCTTGACAATGATACTAGATATCCTCTCTAGGAGTATGAATGATGATGTGATTTCTTG
AGAAGAGAGAAATAAAAAATCAATACAAAGATTTGTTTCTGGACTAGTTTG
   
Sequence Name 04-K119541-0022-322-C04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119541-0022-322-C04C-8409
ACTGTTGTTGTTGATGTTAGGGCAACAACAACAACAACGTTGTGAGGAGGCTTTAACCGA
AATAGATTGTGGTGCTGGCAATTGTAATGCTCTATGCCTACAGAAACGGAAGGGATTGGG
CCGATGCGTCCAAAGAACACCTTGCGATAAATTAAAATGCATGTGTTATTATCCTTGTAG
TTCTTGAATTTAAAATTTTCTTTTCCTATCTATTAATTATCATTAACTAATTAATAAAAT
AGATATTTATTTGCAATCCATTCTGATATGAAATGTGATGAGTCGATCTCAATTATCTCA
AACTAGTCCAGAAACAAATCTTTGTATTGATTTTTATTTTCTCTCTTCTCATGAAATCAC
ATCATCATTCATACTCCTAGAGAGGATATCTAGTATCATTGTCAAGATTAGATAACTTTG
GAGTTTCATCCAATATATGAATATGATAGGCAAAAATGACAATCTCTAATGAGATTTATT
TTGAAGAAAAATAATAATAGTGAATATGGTTCTCGTTTTTGAAAGCGTAAATCTTTTCTA
TATTTATTTATTATATGTGTTGTGTATTGATGATATTAATTAGTGTAATTTGGGTGTATC
AACTTTTTTTTATCATACTTCCAATGAATGAATGAAGTCAATTACCATATATATATATAT
AT

Last Updated on 06.12.2018 16:11