Sequences from confirmation amplicon


Confirmation sequences for line 322D08 and hit At3g48760 are shown.
Line ID 322D08
Locus At3g48760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18077796
Primers used for PCR BV27 - 8474, show primer details
   
Sequence Name 45-K105952-0022-322-D08C-BV27
GenBank Accession LN505536 [GenBank]
Sequencing primer gene specific primer, BV27: 5'-TTGTGTTGTGCTCAACTCTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K105952-0022-322-D08C-BV27
GTCAAGCGGATAATGGATAGTGAGAACATAAACATCTGGAAATCGTTTCTCAAGACTCCT
GCTTCCATTGCACTAATAATCTACACTTTCATTTGCGTCTGGTTTGTTGGTGGGCTTACC
TGTTTCCACTTGTACCTAATGAGTACCAATCAGGTAAAAATCATTACTCTCTAAAAGCTC
CTTGTTTTGAAACTGTTGGATAATTTAGCTACTTGTTAACATTGTTTCTCTGTTGTTTAT
TATGTTGTGATTTGGCAGTCAACTTACGAAAACTTTAGGTATCGGTATGATAGACATGAG
AACCCCTTCAACAAAGGAATAGTGGGGAACTTTATGGAAGTTTTCTGCACAAATGTTGCC
GTATCTCAGAACAGTTTCAGAGAAAAGGTTTCAAAGGAGCCAGCAATACCACCAAGGACA
GTAAATGGAGGGATGTCAAGCCCGAGCTTACAGAAAGTTTCCAACGACATAGAGATGGGG
AGAAAACCAGTGTGGCACGAGACAGTAGAAGAAGAGCTCGGTGACATCGAAAAGGACATG
GAAGCAGGGGTTGCATCACGGGATTTGAGCAGAATGATGGCTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 61-K105953-0022-322-D08C-8409
GenBank Accession LN510525 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K105953-0022-322-D08C-8409
CATTTACAATTGAGCATCATTCTGCTCAAATCCCGTGATGCAACCCCTGCTTCCATGTCC
TTTTCGATGTCACCGAGCTCTTCTTCTACTGTCTCGTGCCACACTGGTTTTCTCCCCATC
TCTATGTCGTTGGAAACTTTCTGTAAGCTCGGGCTTGACATCCCTCCATTTACTGTCCTT
GGTGGTATTGCTGGCTCCTTTGAAACCTTTTCTCTGAAACTGTTCTGAGATACGGCAACA
TTTGTGCAGAAAACTTCCATAAAGTTCCCCACTATTCCTTTGTTGAAGGGGTTCTCATGT
CTATCATACCGATACCTAAAGTTTTCGTAAGTTGACTGCCAAATCACAACATAATAAACA
ACAGAGAAACAATGTTAACAAGTAGCTAAATTATCCAACAGTTTCAAAACAAGGAGCTTT
TAGAGAGTAATGATTTTTACCTGATTGGTACTCATTAGGTACAAGTGGAAACAGGTAAGC
CCACCAACAAACCAGACGCAAATGAAAGTGTAGATTATTAGTGCAATGGAAGCAGGAGTC
TTGAGAAACGATTTCCAGATGTTTATGTTCTCACTATCCATTATCCGCTTGACGTAGATC
CAGCAGAACACATGGACATAAATGCAGAGAAGAG

Last Updated on 06.12.2018 16:11