Sequences from confirmation amplicon


Confirmation sequences for line 323B01 and hit At1g56140 are shown.
Line ID 323B01
Locus At1g56140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21002149
Primers used for PCR EQ22 - 8474, show primer details
   
Sequence Name 43-K102871-0022-323-B01C-EQ22
GenBank Accession LN504797 [GenBank]
Sequencing primer gene specific primer, EQ22: 5'-TACACTGTAAAAGAGACGGCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K102871-0022-323-B01C-EQ22
GAGCTAAAANTATTACCTTAAAAGAACCCAGTGAAATCTAGAGTTTTTCCAATCCACCAA
GAATAAAGTGAACCTCATATACACAAACATAATCTCTATATGACTATATCTATAGCCACC
ATATAGGGTCTTTGTCATCTTCCCTCATTCATCTGGGCTCCAAGCATTGGCCTAGCGTCG
TTGTTTCTCGGTGATATCTCGGATCTAGGCGCCACAAAGCTCGTGAAGGATTCAGAAGCC
TGTGTGTTTCTTAATGGAAAGCCGCTGATTGAGGAGGCTGTGGTGTCATCAAATCTCCAG
TCGGTTAGGTAGCCTGGCTTTGAAGTGACATCACTGACCTCAACATCTCCTGAAAGCATG
GCCACCACTCGTGACATTGGTGGTCTCAAGGCATGAGATGTTTGTGTACACAGCAGGGCT
ATGCCAATCATGCGTTTCCCTTCTTCCATGTTGAATTCAGTCAGCTGATGATCAATAAGT
TCAACTTCACGGCCTTTCTCGTGTAGATTCCATGCCTGTGAACAAGTTACACAATCAATA
TCAGCTTTTGCTAAAGGGCATAACCCAGAGGCTAATCTACATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCGAAAATGTAAATG
   
Sequence Name 43-K102876-0022-323-B01C-8409
GenBank Accession LN504798 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K102876-0022-323-B01C-8409
ATAGCCTCTGGGTATGCCCTTTAGCAAAAGCTGATATTGATTGTGTAACTTGTTCACAGG
CATGGAATCTACACGAGAAAGGCCGTGAAGTTGAACTTATTGATCATCAGCTGACTGAAT
TCAACATGGAAGAAGGGAAACGCATGATTGGCATAGCCCTGCTGTGTACACAAACATCTC
ATGCCTTGAGACCACCAATGTCACGAGTGGTGGCCATGCTTTCAGGAGATGTTGAGGTCA
GTGATGTCACTTCAAAGCCAGGCTACCTAACCGACTGGAGATTTGATGACACCACAGCCT
CCTCAATCAGCGGCTTTCCATTAAGAAACACACAGGCTTCTGAATCCTTCACGAGCTTTG
TGGCGCCTAGATCCGAGATATCACCGAGAAACAACGACGCTAGGCCAATGCTTGGAGCCC
AGATGAATGAGGGAAGATGACAAAGACCCTATATGGTGGCTATAGATATAGTCATATAGA
GATTATGTTTGTGTATATGAGGTTCACTTTATTCTTCTTGGATTGGAAAAACTCTAGATT
TCACTTTGGTTAAATAATAATAATATTTTAGCTCCTCTGACTTCCACAAGCGGTCGTGAT
GGGCCACGTACTATTCACGTCCTCAACCAAATCAATGCCGTCTCTTTTACAGTGT
Primers used for PCR KH55 - 8474, show primer details
   
Sequence Name 80-K118656-0022-323-B01C-KH55
GenBank Accession LN513998 [GenBank]
Sequencing primer gene specific primer, KH55: 5'-GCAAAACTGTACGATGACAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K118656-0022-323-B01C-KH55
ACTCCCAACCCTGACCTCTGGTTTGCAACACAACTATGGATTTATAACACATGTTGGTGA
TTTTACTGTATCAATGCAGTGGGTATCTTGCGCCAGAGTATGCCATGCGTGGACATCTAA
CAGAGAAAACGGATGTATATGCCTTTGGTGTTGTGGCTCTTGAGCTAGTGAGTGGAAGGC
CAAACTCTGATGAGAACTTGGAGGATGAAAAAAGATATCTTCTTGAATGGGTACGTCCAT
ACTTCAACACTTTTTGATAATGAACACAACTTCAATGTATGTGACCATGAGAAAAAGAAA
GAGAAAAAGAAATGGCTAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATATCCGCACGGTTATT
   
Sequence Name 54-K118655-0022-323-B01C-8409
GenBank Accession LN508633 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118655-0022-323-B01C-8409
CCATTTCTTTTTCTCTTTCTTTTTCTCATGGTCACATACATTGAAGTTGTGTTCATTATC
AAAAAGTGTTGAAGTATGGACGTACCCATTCAAGAAGATATCTTTTTTCATCCTCCAAGT
TCTCATCAGAGTTTGGCCTTCCACTCACTAGCTCAAGAGCCACAACACCAAAGGCATATA
CATCCGTTTTCTCTGTTAGATGTCCACGCATGGCATACTCTGGCGCAAGATACCCACTGC
ATTGATACAGTAAAATCACCAACATGTGTTATAAATCCATAGTTGTGTTGCAAACCAGAG
GTCAGGGTTGGGAGTTACTTACATTGTCCCTGCAACCCGGGTACTTATGTGGGTTTTCTT
GTCATCGTAAAGTTTTGC

Last Updated on 06.12.2018 16:11