Sequences from confirmation amplicon


Confirmation sequences for line 324G05 and hit At1g58360 are shown.
Line ID 324G05
Locus At1g58360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21679797
Primers used for PCR CL86 - 8474, show primer details
   
Sequence Name 34-K033099-022-324-G05C-CL86
GenBank Accession LN501313 [GenBank]
Sequencing primer gene specific primer, CL86: 5'-AACTTAGTTCTGGTCTCTGACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K033099-022-324-G05C-CL86
GATTGGAGTATTGTATCACTAACGGTGAAAGTTTTATGTTTTTTGCAGGATACACTAAGA
TCTAGCCCAGCTGAGAACAAAGCCATGAAAAGAGCAAGTCTTGTGGGAGTATCAACCACC
ACTTTTTTCTACATCTTATGTGGATGCATCGGCTATGCTGCATTTGGAAACAATGCCCCT
GGAGATTTCCTCACAGATTTCGGGTTTTTCGAGCCCTTTTGGCTCATTGACTTTGCAAAC
GCTTGCATCGCTGTCCACCTTATTGGTGCCTATCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 82-K033099-022-324-G05C-8409
GenBank Accession LN514134 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K033099-022-324-G05C-8409
TTTACATTGAATATATCCTGATAGGCACCAATAAGGTGGACAGCGATGCAAGCGTTTGCA
AAGTCAATGAGCCAAAAGGGCTCGAAAAACCCGAAATCTGTGAGGAAATCTCCAGGGGCA
TTGTTTCCAAATGCAGCATAGCCGATGCATCCACATAAGATGTAGAAAAAAGTGGTGGTT
GATACTCCCACAAGACTTGCTCTTTTCATGGCTTTGTTCTCAGCTGGGCTAGATCTTAGT
GTATCCTGCAAAAAACATAAAACTTTCACCGTTAGTGATACAATACTCCAATCTTTAATT
TTGGTAAACAGTTTTGAACTAGGTCCATTATTTCCCTGGGTCAGAGACCAAAACTAAG

Last Updated on 06.12.2018 16:11