Sequences from confirmation amplicon


Confirmation sequences for line 325B05 and hit At5g62520 are shown.
Line ID 325B05
Locus At5g62520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25098832
Primers used for PCR JK36 - 8474, show primer details
   
Sequence Name 15-K026796-022-325-B05C-8409
GenBank Accession LN492815 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K026796-022-325-B05C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAAGAAACTCAGGCAAAACATGAGTGTT
CATGTGTGTACTCCACACAATATACTTCTTCGTAGAAACTAAATCATCCACACCAGAATC
AAACTCTGGTGAACTTGGACATGATCTAGTCGAGCCTTGAGGAACAATCTCTGATTTTCC
AAGTAGAACTCTACAAAGCAACAAGAATCTCATCCCATCTTCTGATTCTGAAGCTGAATC
CTTCAAACTAATCAAACAAACAAACACCAAAAATGAGATTTTGAGACCAATGAATTTCGA
AAAATTGAAACTTTTAGAGATTTAGAGAAACTTACCAATCAAGAGGAGAATTATCCGGCG
AGAGATACAATCCACGGCCAAAAGATCCATCATTCCTCAGCGGCTCGCTAAACCCATACT
CAAAAATCGTTTTAAGCTCATGTTTCGAAACAGAGCACCAACCGTATTTCACCTTAGCTC
CACCGTCTCCACCGTGTTTCATCTGCACCGCCTCTTGAAAAACCTGAAAAGTCTTGAGCT
TTGCACGAGATCCAACGTTCCTAAATCCGTTTCTAAGAACAGAGAGAATCTG
   
Sequence Name 16-K026796-022-325-B05C-JK36
GenBank Accession LN493283 [GenBank]
Sequencing primer gene specific primer, JK36: 5'-TGGTGATCAATGTCAGATTCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K026796-022-325-B05C-JK36
TTCTTAGAAACGGATTTAGGAACGTTGGATCTCGTGCAAAGCTCAAGACTTTTCAGGTTT
TTCAAGAGGCGGTGCAGATGAAACACGGTGGAGACGGTGGAGCTAAGGTGAAATACGGTT
GGTGCTCTGTTTCGAAACATGAGCTTAAAACGATTTTTGAGTATGGGTTTAGCGAGCCGC
TGAGGAATGATGGATCTTTTGGCCGTGGATTGTATCTCTCGCCGGATAATTCTCCTCTTG
ATTGGTAAGTTTCTCTAAATCTCTAAAAGTTTCAATTTTTCGAAATTCATTGGTCTCAAA
ATCTCATTTTTGGTGTTTGTTTGTTTGATTAGTTTGAAGGATTCAGCTTCAGAATCAGAA
GATGGGATGAGATTCTTGTTGCTTTGTAGAGTTCTACTTGGAAAATCAGAGATTGTTCCT
CAAGGCTCGACTAGATCATGTCCAAGTTCACCAGAGTTTGATTCTGGTGTGGATGATTTA
GTTTCTACGAAGAAGTATATTGTGTGGAGTACACACATGAACACTCATGTTTTGCCTGAG
TTTCTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATG

Last Updated on 06.12.2018 16:11