Sequences from confirmation amplicon


Confirmation sequences for line 325C11 and hit At2g01210 are shown.
Line ID 325C11
Locus At2g01210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:120317
Primers used for PCR EQ23 - 8474, show primer details
   
Sequence Name 41-K102871-0022-325-C11C-EQ23
GenBank Accession LN504037 [GenBank]
Sequencing primer gene specific primer, EQ23: 5'-GATCCCATAAGAGTAAACATCCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K102871-0022-325-C11C-EQ23
GTTTCATCTTGAGAGTCTCAGGTGCTTGATAGTAGGATCCAGAAGAAGAGTGAGCAGTGA
ACTCCGAAGATACGCTCTTGTGATGGTGTTGTTGCCTCTCTTGCGGCTGTTGATCCGTTT
GGATGATTCTGTTAGACTGTATGGTCGGAGAAGATCCTCCAGCGATGTTAGCTAACCGTG
CTAGTCCAAAATCTGAGATCTTAGGTTCCATGTCCTGTCCAATGAGAATGTTGCTTGGCT
TGAGATCTCCATGGACGTATTTCTTGGGACTAAACTCATGCAGATAAACAAGCCCTGTAG
CAATCCCTTTCATTATCCTCAACCGTTCAGACCATGTCAACGGAGCGATGGTCATCATCC
CCGGCTTTCCTGCACATACACACACACACATTTACCATGATGTAACTGAATAATGCATCT
TGAAATGAGAAAAGAGTGACTCATCGTGGAGGGCGGTTGCGAGGTTACCGTTGGAAACAT
AGTCCATTTTCCATTGTAAATGGCTTCATGTCCAGGAAATCTAACTGGATCAGCATTGAG
TATGA
   
Sequence Name 41-K102876-0022-325-C11C-8409
GenBank Accession LN504038 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K102876-0022-325-C11C-8409
CGGACTGAGCCATTTACATTGAAAATGGACTATGTTTCCACGGTAACCTCGCAACCGCCC
TCCACGGTGAGTCACTCTTTTCTCATTTCAAGATGCATTATTCAGTTACATCATCGTAAA
TGTGTGTGTGTGTATGTGCAGGAAAGCCGGGGATGATGACCATCGCTCCGTTGACATGGT
CTGAACGGTTGAGGATAATGAAAGGGATTGCTACAGGGCTTGTTTATCTGCATGAGTTTA
GTCCCAAGAAATACGTCCATGGAGATCTCAAGCCAAGCAACATTCTCATTGGACAGGACA
TGGAACCTAAGATCTCAGATTTTGGACTAGCACGGTTAGCTAACATCGCTGGAGGATCTT
CTCCGACCATACAGTCTAACAGAATCATCCAAACGGATCAACAGCCGCAAGAGAGGCAAC
AACACCATCACAAGAGCGTATCTNCGGAGTTCACTGCTCACTCTTCTTCTGGATCCTACT
ATCANGCACCTGAGACTCTCAAGATGGTAAAACCTTCACAGAAGTGGGATGTTT
Primers used for PCR KH56 - 8474, show primer details
   
Sequence Name 79-K118656-0022-325-C11C-KH56
GenBank Accession LN513881 [GenBank]
Sequencing primer gene specific primer, KH56: 5'-GAAACCAAACAGAAATCAAGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K118656-0022-325-C11C-KH56
TTGGTATCTGCCTTGTGGGTTTGCTTTTCACTTACTGCTACTCAAAGTTCTGCGCTTGTA
ACCGCGAGAACCAATTCGGAGTCGAGAAAGAATCCAAGAAAAGGGCTTCTGAGTGTCTGT
GTTTCCGTAAAGACGAATCCGAAACGCCATCTGAAAACGTGGAGCATTGCGACATTGTTC
CCCTCGACGCTCAGGTTGCGTTTAACCTGGAGGAGCTGCTAAAGGCGTCAGCTTTCGTTC
TGGGGAAGAGCGGAATCGGAATTGTCTACAAAGTGGTTCTAGAGAACGGGCTCACACTGG
CCGTACGGAGATTGGGTGAAGGAGGGTCTCAGAGATTCAAGGAGTTTCAGACAGAAGTTG
AAGCCATAGGGAAACTAAAACATCCTAACATTGCTAGTCTTCGAGCTTATTATTGGTCTG
TCGATGAAAACACAGTGAATGTGTGTCACTCTTTTCTCATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATATAGATGTCCGCA
   
Sequence Name 53-K118655-0022-325-C11C-8409
GenBank Accession LN508308 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118655-0022-325-C11C-8409
ATTTCATGAGAAAAGAGTGACACACATTCACTGTGTTTTCATCGACAGACCAATAATAAG
CTCGAAGACTAGCAATGTTAGGATGTTTTAGTTTCCCTATGGCTTCAACTTCTGTCTGAA
ACTCCTTGAATCTCTGAGACCCTCCTTCACCCAATCTCCGTACGGCCAGTGTGAGCCCGT
TCTCTAGAACCACTTTGTAGACAATTCCGATTCCGCTCTTCCCCAGAACGAAAGCTGACG
CCTTTAGCAGCTCCTCCAGGTTAAACGCAACCTGAGCGTCGAGGGGAACAATGTCGCAAT
GCTCCACGTTTTCAGATGGCGTTTCGGATTCGTCTTTACGGAAACACAGACACTCAGAAG
CCCTTTTCTTGGATTCTTTCTCGACTCCGAATTGGTTCTCGCGGTTACAAGCGCAGAACT
TTGAGTAGCAGTAAGTGAAAAGCAAACCCACAAGGCAGATACCAAACACGTCGCAGAGAA
CAATGGCAATGACGGCGCTTTTGCTCAAACCACTTGATTTCGNTTTGGTTT

Last Updated on 06.12.2018 16:11