Sequences from confirmation amplicon


Confirmation sequences for line 326A01 and hit At5g67385 are shown.
Line ID 326A01
Locus At5g67385 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26886353
Primers used for PCR BH57 - 8474, show primer details
   
Sequence Name 11-K104736-0022-326-A01C-BH57
GenBank Accession LN491100 [GenBank]
Sequencing primer gene specific primer, BH57: 5'-CTGATGGTTGATGATGATGTTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104736-0022-326-A01C-BH57
TTTACTTCATTTTATTTTGAGAGTTCGAGCTTCAGTCCTGATTTTCGCGTTTAAGCTTGG
AGAGTTCGTCGGTGTAGGAGCTAAGTTTGGGAGGAAGAACAGCTGCTGGTTGTGGTGGTG
GTGGGGCTGGAGAATCTGAATCGTTAGTGACTTCTCCTCGTAGACGTTGTTGCTCGTAGT
AAAGGACTTGAACAATGGTCTGAACAGGAAGACGATCATTCTGAGCTGCATGAGCGCAGG
CTTCTCGTGATAGTTTTTGGCAGTCCATTAAGCTGCACACTTTCTTCCTCTCTACATCAC
TCATATTGGGATGCGCCTGCAAGAAAAAACCATAGTTGTTCACTAGAGCTCTCTTAAAGT
TTTTCAAAATCTCTAAGATAATGGGAACTTGATCATCTCCTTAACCACAAGATGTCAAAT
ATTTACCAGTTTTTGTAGTCTCATCTAAACGGATTCGGTATAGAGCAAATGAATGTAAAT
GTTTACTAACTTAATATTACCATCAATATGATGTCAATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTA
   
Sequence Name 27-K104737-0022-326-A01C-8409
GenBank Accession LN498372 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104737-0022-326-A01C-8409
GCCTTTACAATTGAATTGACATCATATTGATGGTAATATTAAGTTAGTCAATATTTACAT
TCATTTGCTCTATACCGAATCCGTTTAGATGAGACTACAAAAACTGGTAAATATTTGACA
TCTTGTGGTTAAGGAGATGATCAAGTTCCCATTATCTTAGAGATTTTGAAAAACTTTAAG
AGAGCTCTAGTGAACAACTATGGTTTTTTCTTGCAGGCGCATCCCAATATGAGTGATGTA
GAGAGGAAGAAAGTGTGCAGCTTAATGGACTGCCAAAAACTATCACGAGAAGCCTGCGCT
CATGCAGCTCAGAATGATCGTCTTCCTGTTCAGACCATTGTTCAAGTCCTTTACTACGAG
CAACAACGTCTACGAGGAGAAGTCACTAACGATTCAGATTCTCCAGCCCCACCACCACCA
CAACCAGCAGCTGTTCTTCCTCCCAAACTTAGCTCCTACACCGACGAACTCTCCAAGCTT
AAACGCGAAAATCAGGACTTGAAGCTCGAACTTCTCAAAATGAAAATGAAGCTAAAAGAG
TTTGAGAAAGAGAGTGAGAAGAAAACATCATCATCAACCATCA

Last Updated on 06.12.2018 16:11