Sequences from confirmation amplicon


Confirmation sequences for line 326B11 and hit At1g29330 are shown.
Line ID 326B11
Locus At1g29330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10259160
Primers used for PCR TU04 - 8474, show primer details
   
Sequence Name 03-K026965-022-326-B11C-8409
GenBank Accession LN487335 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K026965-022-326-B11C-8409
GTAGATTTCCCGGACATGAAGCCATTTAAAAATCAGTGCATATTTCTAAAGTCCAGACAT
TGTTTTCCGTTTAATGTTAATGCGTCTGGAAGCTGAGCTGAACTATATTTTTTTCTATAA
AGGAAATCAGACTGCTTTACATCTTGCTATGCATTTGGTTCTGATGCATACCTTTGTGTC
CAGTGCACAATATTTCAATGTGCAAAATTGAGTAAGTATTCTGTATGCACTCACATGGGC
AATGATTAACATGCAGGGCGTATCGGGGACTCTATATTATCAACTGGATCTATCGCTATT
TCACAGAAGATCATTTCACTAGATGGATTGGTAATGTTTCAACAAAGAAGCACTAAATGC
ATTTTAACCATTTGTTTTTCCTCAATGCTAATGTTTTACGTAAATCCATTTTCCAGCTTG
TGTGTCTGGTCTTGTCCAAACAGCTCTCTATGCGGATTTCTTCTACTACTACTACATAAG
GTAAAGTAACCTCACCAGTCTGAATAATATTCTCTTTATAGTTACTGATCATGTTACTGA
CTTTGGATCCTTTTTGATTTTGTTGTAGCTGGAAAACCAACACCAAACTTAAGCTTCCGG
CTTGAAAAGAACCCGAATTTTTTTGCAATAGTATCAAAA
   
Sequence Name 04-K026965-022-326-B11C-TU04
GenBank Accession LN487773 [GenBank]
Sequencing primer gene specific primer, TU04: 5'-ATTGCTGTAACTTCCTGGATTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K026965-022-326-B11C-TU04
CTATGCAAAAAAATTCGGGTTCTTTTCAAGCCGGAAGCTTAAGTTTGGTGTTGGTTTTCC
AGCTACAACAAAATCAAAAAGGATCCAAAGTCAGTAACATGATCAGTAACTATAAAGAGA
ATATTATTCAGACTGGTGAGGTTACTTTACCTTATGTAGTAGTAGTAGAAGAAATCCGCA
TAGAGAGCTGTTTGGACAAGACCAGACACACAAGCTGGAAAATGGATTTACGTAAAACAT
TAGCATTGAGGAAAAACAAATGGTTAAAATGCATTTAGTGCTTCTTTGTTGAAACATTAC
CAATCCATCTAGTGAAATGATCTTCTGTGAAATAGCGATAGATCCAGTTGATAATATAGA
GTCCCCGATACGCCCTGCATGTTAATCATTGCCCATGTGAGTGCATACAGAATACTTACT
CAATTTTGCACATTGAAATATTGTGCACTGGACACAAAGGTATGCATCAGAACCAAATGC
ATAGCAAGATGTAAAGCAGTCTGATTTCCTTTATAGAAAAAAATATAGTTCAGCTCAGCT
TCCAGACGCATTAACATTAAACGGAAAACAATGTCTGGACTTTAGAAATATGCACTGATT
TTTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCACAT
ATGGAGAANAAGAAAGAGTAATTACCAATTT

Last Updated on 06.12.2018 16:11