Sequences from confirmation amplicon


Confirmation sequences for line 326C05 and hit At3g57650 are shown.
Line ID 326C05
Locus At3g57650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21351889
Primers used for PCR 07ie - 8474, show primer details
   
Sequence Name 33-K112875-0022-326-C05C-8409
GenBank Accession LN501066 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K112875-0022-326-C05C-8409
GCCATTTACTGACAGTGACTATTCCAAAAACCTCTCCACCACCCTCGATGCTAAGACTAT
TCAAAGGACAACCTTCAGTGGTAAGCAAGCAAAAATATGAATAGGTCGTATAGCAGTTGA
ATCTTGCACTCTTCTCACAACAAATTTCAAAGTATGCACTGGCTTGGATCACATCACCAT
CCAATTTACTGAAACGAACATAACTCTTTATATCACCTTTACTTTTATAATGTGTAAGAA
ACCGTAGGACTCATCCCATCCCAAGCCTTTTTCTTTTAAAACCTCCTGCGCCATTAACGC
AAACCATTGCTGCTGGTTTCAAGGTGCATGTTCACATCAAGTGTCACTCGATGAAAGACT
TACCTGAATCAGATGACGCANTTGCACAGTGGTGCAGAGATCAGTTTGTGGCTAAGGTAA
AAATTATGGATGTACTGGTATTTCCACGTTTCAGTTATTGGCAATAATATATTTGGTCAA
GATTTGATAGAGAAAAGCTGGGAAAAG
   
Sequence Name 49-K112876-0022-326-C05C-07ie
GenBank Accession LN506899 [GenBank]
Sequencing primer gene specific primer, 07ie: 5'-GCTTTTCCAAGCTTTTCTCTATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K112876-0022-326-C05C-07ie
GCATACTGAACGTGGAAATCCAGTACATCCATAATTTTTACCTTAGCCACAAACTGACCT
TTGCACCACTGTGCAATTGCGTCATCTGATTCAGGTAAGTCTTTCATCGAGTGACACTTG
ATGTGAACATGCACCTTGAAACCAGCAGCAATGGTTTGCGTTAATGGCGCAGGAGGTTTT
AAAAGAAAAAGGCTTGGGATGGGATGAGTCCTACGGTTTCTTACACATTATAAAAGTAAA
GGTGATATAAAGAGTTATGTTCGTTTCAGTAAATTGGATGGTGATGTGATCCAAGCCAGT
GCATACTTTGAAATTTGTTGTGAGAAGAGTGCAAGATTCAACTGCTATACGACCTATTCA
TATTTTTGCTTGCTTACCACTGAAGGTTGTCCTTTGAATAGTCTTAGCATCGTGGGTGGT
GGAGAGGTTTTTGGAATAGTCACTGTCATGTAAATGGCTTCATGTCCGGACCAAAACCTA
CATGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCATTATT

Last Updated on 06.12.2018 16:11