Sequences from confirmation amplicon


Confirmation sequences for line 326F07 and hit At2g34210 are shown.
Line ID 326F07
Locus At2g34210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14449823
Primers used for PCR 19vf - 8474, show primer details
   
Sequence Name 55-K117939-0022-326-F07C-19vf
GenBank Accession LN508931 [GenBank]
Sequencing primer gene specific primer, 19vf: 5'-AATACTTGCCTCCAAGAATCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K117939-0022-326-F07C-19vf
TCTGTGTCGCTCTTATCGTCTCGCCTTCACCTCTGTGTCCCATTGCGACTACACAGGAGC
CATCCTGATGATGACCACAAAGGAAAAAGAGGCTTACATAATCAGTAAACTGGAAAATGT
TTACTCCAATACAACATTCTTCTGTGTAGTTTAAAGAGGACAAACCGGAAGAACATCTCG
AATAACACCGGGATTTCTATCCTCTCCAGCTTTGTGTACATTGACCAATATACCGGGTAT
GAACCTGGTTTCTGCATCTCCACCTGGTAGAGTTAAGAATATGAGAATCAAAAACACCAA
TGGTATAAAACTAAGAGATAGGCAGGCACCTATATCAAGAGACATGACATCGAGATCGGT
TCCAGGAGTCATTGCTTGTCCTCCGGGTGTGGTAGGTAAGTAGGAAGTTGAGCTTGGTGT
CATAGGACTTGGGGCATTAGCTGCATGAGAAAAGTGTGTGAGAATAAGATTCGTCAAAGC
AAGAAACCGTTAAGGCATAAAAGGTAGTAGACAAAACCTGGTCGTAGTAGTTACAAGGCA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGGTTATT
   
Sequence Name 46-K117938-0022-326-F07C-8409
GenBank Accession LN505998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K117938-0022-326-F07C-8409
ATTGAATAATGCCTTGTACTACTACGACCAGGTTTTGTCTACTACCTTTTATGCCTTAAC
GGTTTCTTGCTTTGACGAATCTTATTCTCACACACTTTTCTCATGCAGCTAATGCCCCAA
GTCCTATGACACCAAGCTCAACTTCCTACTTACCTACCACACCCGGAGGACAAGCAATGA
CTCCTGGAACCGATCTCGATGTCATGTCTCTTGATATAGGTGCCTGCCTATCTCTTAGTT
TTATACCATTGGTGTTTTTGATTCTCATATTCTTAACTCTACCAGGTGGAGATGCAGAAA
CCAGGTTCATACCCGGTATATTGGTCAATGTACACAAAGCTGGAGAGGATAGAAATCCCG
GTGTTATTCGAGATGTTCTTCCGGTTTGTCCTCTTTAAACTACACAGAAGAATGTTGTAT
TGGAGTAAACATTTTCCAGTTTACTGATTATGTAAGCCTCTTTTTCCTTTGTGGTCATCA
TCAGGATGGCTCCTGTGTAGTCGCACTTGGACACAGAGGTGAAGGCGAGACGATAAGAGC
GACACAGAACAAAGTGAGCCTAGTTTGTCCGAAGAAGAACGAACGTGTGAAGATTCTTGG
AGCCCAGTATT

Last Updated on 06.12.2018 16:11