Sequences from confirmation amplicon


Confirmation sequences for line 326G10 and hit At2g26770 are shown.
Line ID 326G10
Locus At2g26770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11406073
Primers used for PCR 05md - 8474, show primer details
   
Sequence Name 50-K114180-0022-326-G10C-05md
GenBank Accession LN507246 [GenBank]
Sequencing primer gene specific primer, 05md: 5'-CATGCAGTTTTTGTTAGTCCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K114180-0022-326-G10C-05md
TTTTGTTTTCTTTGAGTAGTTATATTCTATTACTTTTGACAAAGAACTATGATGTAGTTT
CTTTACAGACATAATCTTTCACTTACTATTCTTGATTCTTTTTCGTATCTTGCTTGGGAC
ATTTGTATTACTCTTCTGTTGTCGGTTGTAAATTAGTGGTTACTAGATGGGCTAGCTTGA
ATAAGATGCAAATGTGTTCTGATAATCTTGCTGATTAGTGGGTATGATTTTTTAACTAGG
CCTCAGAGGATGCTAAGAAACTTGTCGACCAGGAAAGAGCGTTTGCACGTGCTGAGATAG
AAAGTGCAAGAGCAGCTGTACTAAGAGTTGAAAAAGCCCTGCGAGAACATGAACAGATGT
CTCGTGCCTCTTGGAAACAAATATTTCAGTGATGGCTTCTATCAAAATCTTGTAAATGTC
CTATGCTTATAACATTCAATTGAAGATGGATTCTTGTCCAGGAAATCTGCATGGATCAGG
CAATGAGTAGGATGGTCGATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA
   
Sequence Name 14-K114177-0022-326-G10C-8409
GenBank Accession LN492597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114177-0022-326-G10C-8409
ATTTTCATTGAATGTTAAAGCATAGGACATTTACAAGATTATGATAGAAGCCATCACTAA
AATACTTGTTTCCCAGAGGCACGAGACATCTGTTCATGTTCTCGCAGGGCTTCTTCAACT
CTTTGTACAGCTGCTCTTGCACTTTCAATCTCAGCACGTGCAAACGCTCTTTCCTCGTCG
ACAAGTTTCTTAGCATCCTCTGAGGCCTAGTTAAAAAATCATACCCACTAATCAGCAAGA
TTATTAGAACACATCTGCATCTTTTTCAAGCTAGCCCATCTAGTAACCACTAATTTACAA
CCGACAACAGAAGACTAATACAAATGTCCCAAGCAAGATAAGAAAAAGAATCAAGAATAG
TAAGTGAAAGATTATGTCTGTAAAGAAACTACATCATAGTTCTTTGTCAAAAGTAATAGA
ATATAAACTACTCAAATGAAAACAAAAAGGACAGACTCTCTCTTAATCATCTGGTTAGCT
CAAGGACTAACAAAAACTGCAT

Last Updated on 06.12.2018 16:11