Sequences from confirmation amplicon


Confirmation sequences for line 326H06 and hit At2g24800 are shown.
Line ID 326H06
Locus At2g24800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10571429
Primers used for PCR GD84 - 8474, show primer details
   
Sequence Name 63-K033741-022-326-H06C-GD84
GenBank Accession LN510921 [GenBank]
Sequencing primer gene specific primer, GD84: 5'-CCGATTTCACAGTTGATAAGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K033741-022-326-H06C-GD84
GGCTATCTGTTCTGATCTAGTTGTCCTCTCGGTACGTTATCTTTCTTGATACTCAAGAAA
GAGAGATCTTTCTTTAAAACAAACCTTCAATTTTCGATCTATTACGGGTCTCAGGAGCGC
ACACTATTGGAGCTGCGCATTGTAACACGTTCAACAGCAGGTTTAAGCTTGATCCAAAAG
GCAACTTAGAACTAATAGACGCGTCTCTCGATAACTCCTATGCGCAAACACTTGTAAACA
AGTGTTCATCGTCACTAGATCCAACGACCACGGTGGTTGATAACGATCCAGAGACATCGT
CGACGTTCGATAATCAGTACTACAAGAATCTGTTGGCTCACAAGGGTTTGTTTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CA
   
Sequence Name 53-K033741-022-326-H06C-8409
GenBank Accession LN508067 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K033741-022-326-H06C-8409
CGGACTGAGCCATTTACATTGAAACAAACCCTTGTGAGCCAACAGATTCTTGTAGCTACT
GATTATCGAACGTCGACGATGTCTCTGGATCGTTATCAACCACCGTGGTCGTTGGATCTA
GTGACGATGAACACTTGTTTACAAGTGTTTGCGCATAGGAGTTATCGAGAGACGCGTCTA
TTAGTTCTAAGTTGCCTTTTGGATCAAGCTTAAACCTGCTGTTGAACGTGTTACAATGCG
CAGCTCCAATAGTGTGCGCTCCTGAGACCCGTAATAGATCGAAAATTGAAGGTTTGTTTT
AAAGAAAGATCTCTCTTTCTTGAATATCAAGAAAGATAACGTACCTGAGAGGACAACTAG
ATCATGAACAGATAAGCCTTTAGAGGAAAAGATATTGATCATCTTATCAACTGTGNAAAT
CGG
Primers used for PCR GD85 - 8474, show primer details
   
Sequence Name 64-K033741-022-326-H06C-GD85
GenBank Accession LN511134 [GenBank]
Sequencing primer gene specific primer, GD85: 5'-GATACATTAACGTTTGACGGGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K033741-022-326-H06C-GD85
CACTCAAATGCATACTTTGTGGAGATGATATTGCTATCTGTTTGTTTTTAAAAAAATACT
TTTCATTAAGGTTCGAATCCATCTTAAAACACGCATAAAAGATCGATCCATGTGGCAATA
TCCTAAATGGTTCAAAAAAAACGCATGAAGTTCCTTAACAGTAAAATTTACATTTTGCAA
CTCCTTCTGTATAAAATTACTAATATTTAATCTTTTTTAAATATTCGTATTGTCTACATT
GGAGTTGAAAATAAATAAATCAAGAAATTGTTGATTAGGAGAAACTTGGTCTAATCTTCG
TTTAATTAACAGCAGAACAAGAACGTCTAATCTCACCTTCTTCACCAACTCTCACTCCCA
TCAAACTCATCTTCAAAAAAGACTCAGTCCATCTATCGAAAAAACTCTCCTGATCATTCG
CAAGAATCTCAACAATCTTCCTCGTCCGATCATCTTCCATGAGTATGATGGATCAGCAAT
GAGTATAAAATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 54-K033741-022-326-H06C-ORIL
GenBank Accession LN508392 [GenBank]
Sequencing primer gene specific primer, ORIL: unknown
Sequence (A. th genome BLAST matches underlined) 
>54-K033741-022-326-H06C-ORIL
TTTATACTCATTGCTGATCCATCATACTCATGGAAGATGATCGGACGAGGAAGATTGTTG
AGATTCTTGCGAATGATCAGGAGAGTTTTTTCGATAGATGGACTGAGTCTTTTTTGAAGA
TGAGTTTGATGGGAGTGAGAGTTGGTGAAGAAGGTGAGATTAGACGTTCTTGTTCTGCTG
TTAATTAAACGAAGATTAGACCAAGTTTCTCCTAATCAACAATTTCTTGATTTATTTATT
TTCAACTCCAATGTAGACAATACGAATATTTAAAAAAGATTAAATATTAGTAATTTTATA
CAGAAGGAGTTGCAAAATGTAAATTTTACTGTTAAGGAACTTCATGCGTTTTTTTTGAAC
CATTTAGGATATTGCCACATGGATCGATCTTTTATGCGTGTTTTAAGATGGATTCGAACC
TTAATGAAAAGTATTTTTTTAAAAACAAACAGATAGCAATATCATCTCCACAAAGTATGC
ATTTTGAGTTGTAATAAGTAATAACGAATTTCAAAAATAAACTCCCGTCAAACTAAAATG
TAT

Last Updated on 06.12.2018 16:11