Sequences from confirmation amplicon


Confirmation sequences for line 327B12 and hit At1g35720 are shown.
Line ID 327B12
Locus At1g35720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13225186
Primers used for PCR SP03 - 8474, show primer details
   
Sequence Name 23-K025446-022-327-B12C-8409
GenBank Accession LN496408 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K025446-022-327-B12C-8409
CCGGACATGAAGCCATTTACAATTGAATATAATTCATGATTTGGTATATACGGACAGGCC
ACGTCGTGTCCTAAACCTCTTAGCCTTTCCCTTTATAAGTCAATCTTGTGTCGGCTTCGA
CTCCCAACATACACAAAACACTAAAAGTAGAAGAAAAATGGCGACTCTTAAGGTTTCTGA
TTCTGTTCCTGCTCCTTCTGATGATGCTGAGCAATTGAGAACCGCTTTTGAAGGTATAAT
AGTTTCATGATTCTTCTTGTTACGTTCTCTGTTTTTGTTACCTTCTTGTTTACATGATCA
GTATCCACTTTTTGTAAAAGTGATGTGAATCTGTTGGTTTTCGTTTGGTGGGTTATTGTA
TGATCATTTGTTTTGATTGTTTTTCATGTGGATCGATCTTTGTTTTGGTCCGATGATATG
   
Sequence Name 24-K025446-022-327-B12C-SP03
GenBank Accession LN496848 [GenBank]
Sequencing primer gene specific primer, SP03: 5'-GACACAACATTCATATCATCGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K025446-022-327-B12C-SP03
AAACAAAGATCGATCCACATGAAAAACAATCAAAACAAATGATCATACAATAACCCACCA
AACGAAAACCAACAGATTCACATCACTTTTACAAAAAGTGGATACTGATCATGTAAACAA
GAAGGTAACAAAAACAGAGAACGTAACAAGAAGAATCATGAAACTATTATACCTTCAAAA
GCGGTTCTCAATTGCTCAGCATCATCAGAAGGAGCAGGAACAGAATCAGAAACCTTAAGA
GTCGCCATTTTTCTTCTACTTTTAGTGTTTTGTGTATGTTGGGAGTCGAAGCCGACACAA
GATTGACTTATAAAGGGAAAGGCTAAGAGGTTTAGGACACGACGTGGCCTGTCCGTATAT
ACCAAATCATGAATTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11