Sequences from confirmation amplicon


Confirmation sequences for line 328A03 and hit At5g02150 are shown.
Line ID 328A03
Locus At5g02150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:425441
Primers used for PCR GD79 - 8474, show primer details
   
Sequence Name 49-K033709-022-328-A03C-8409
GenBank Accession LN506746 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K033709-022-328-A03C-8409
CCTTGTACTTCTTTGAATGTATGGGCAGATCTTCATTCCGTTGGAGGCTTGGTACCTCTT
CTTGGCTACCTCAAAAACTCCAATGCTAATATCCGGGCTAAGTCTGCAGATGTTGTAAGC
ACAATAGTTGAGAACAATCCTCGGAGTCAAGAATCAGTTATGGAAGCCAATGGTTTAGAG
TCCTTGCTTTTAAGGTTTACTTCAGACACCGATATGCATTCTCGAACACAGGCTCTCGGT
GCAATATCATGTAAGCTGCTCTGAGTTCTATCCTATATACTTAGATGGTCGAATGCCTTT
TTCAAAAACAAAAATGTCTATCGTAAATCTGATGAATGTATTGTGGTAAATTTGGGGTAC
AGCTTTGATTCGCAATAATAAACCTGGGATCACAGGTTTCCGGATTGCAAATGGTTACTC
TGGTTTAAAAGATGCTTTGGAAACTGATAGTGTGAGATTTCAAAGGTAATAATACTCTTC
ACTTTTCTTCATGCTTGGATTCCTCTGAACTTTGAGAGAACACAAAGTTGTAACCTTTGT
TCTGCTGTTGTCTAACTTTCAAAACTTTTGTCATTACTTTCTCATTCTTCAGGAAAGCTT
TAAACTTGTTGCATTATCTTCTACAAGAGAATGACTCAGACTCCGATATTGCTATAGAAT
TCGGGCTTCACCATTTGATGATGCACCTCGTGTCTAGCTTCGATGCTGATGTAAGAGAAG
CTGCTCTTCGAGGATTGCTTGAGCTCGTGAAAGCTAGAAAAGACTGTAGTACTTGTGGTT
CTAGCATAGTGAAAGGCGATGAGAGACTCAGACAGATTCTAAAAGATCGGATCAAAGCAA
TCAGCCGGGTCAAAGCAATGAGCTTGTTTATGTCACAAGAAGATCTATCAGCCGCTAAAG
AG
   
Sequence Name 65-K033709-022-328-A03C-GD79
GenBank Accession LN511332 [GenBank]
Sequencing primer gene specific primer, GD79: 5'-ATCTAAGAGCTGCCTCTCCTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K033709-022-328-A03C-GD79
CAGCTCATTGCTTTGACCCGGCTGATTGCTTTGATCCGATCTTTTAGAATCTGTCTGAGT
CTCTCATCGCCTTTCACTATGCTAGAACCACAAGTACTACAGTCTTTTCTAGCTTTCACG
AGCTCAAGCAATCCTCGAAGAGCAGCTTCTCTTACATCAGCATCGAAGCTAGACACGAGG
TGCATCATCAAATGGTGAAGCCCGAATTCTATAGCAATATCGGAGTCTGAGTCATTCTCT
TGTAGAAGATAATGCAACAAGTTTAAAGCTTTCCTGAAGAATGAGAAAGTAATGACAAAA
GTTTTGAAAGTTAGACAACAGCAGAACAAAGGTTACAACTTTGTGTTCTCTCAAAGTTCA
GAGGAATCCAAGCATGAAGAAAAGTGAAGAGTATTATTACCTTTGAAATCTCACACTATC
AGTTTCCAAAGCATCTTTTAAACCAGAGTAACCATTTGCAATCCGGAAACCTGTGATCCC
AGGTTTATTATTGCGAATCAAAGCTGTACCCCAAATTTACCACAATACATTCATCAGATT
TACGATAGACATTTTTGTTTTTGAAAAAGGCATTCGACCATCTAAGTATATAGGATAGAA
CTCAGAGCAGCTTACATGATATTGCACCGAGAGCCTGTGTTCGAGAATGCATATCGGTGT
CTGAAGTAAACCTTAAAAGCAAGGACTCTAAACCATTGGCTTCCATAACTGATTCTTGAC
TCCGAGGATTGTTCTCAACTATTGTGCTTACAACATCTGCAGACTTAGCCCGGATATTAG
CATTGGAGTTTTTGAGGTAGCCAAGAAGAGGTACCAAGCCTCCAACGGAATGAAGATCTG
CCCATACATTCAAAGAAGTACAAGGTTACATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAAGAAGAGTAATTACCATTTTTTTTTC
ATTCAAAAATGTAGATGTCCGCAGCGTTTATATAAAATGAAAGTACATTTTGAAAAACGA
CAAATA

Last Updated on 06.12.2018 16:11