Sequences from confirmation amplicon


Confirmation sequences for line 328A11 and hit At1g32960 are shown.
Line ID 328A11
Locus At1g32960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11945513
Primers used for PCR HM54 - 8474, show primer details
   
Sequence Name 41-K101219-0022-328-A11C-8409
GenBank Accession LN504010 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K101219-0022-328-A11C-8409
TTCGGACTGAGCCATTTACATTGAATATATAACAACCAAAACTTGGTCATAAGTCATAAC
CTATTCTCTTTTTTCGGTCTGAGAAAAAAAGAACACTTTACCTTGCTCTCAGTCTCAGAA
CGAGCTCGTGTGGCATTCAGAACTGTTATAAGACTAAGAAGCACCAAGAGAATGGATGAT
CTGAAACTCCTCATTCCAGAAAAGAAATAGTGTTCCTTCACTTTCTTTCAATCTACTTGA
ATATTTTTAAAGAAGCACCAGCATTTATATATATATAGAAACGTGTTTTAGTTTGTATGC
AATTATACTACGTACGACTTGTGGGAGACTGAGACGAAAATTCAAAATAAACGGAAATGT
CTTGGTCTTTGTGTGGCCAAGAATGTTTTGTTTTCTTTGTGGGGTCTAATGTATTCTAGA
TGAACAAGATATAATAATTTCAAAGTATCTGCAGCACTTTCCTTACTATGTTC
   
Sequence Name 41-K101226-0022-328-A11C-HM54
GenBank Accession LN504011 [GenBank]
Sequencing primer gene specific primer, HM54: 5'-GGAACATAGTAAGGAAAGTGCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K101226-0022-328-A11C-HM54
ATTAGACCCCACAAAGAAAACAAAACATTCTTGGCCACACAAAGACCAAGACATTTCCGT
TTATTTTGAATTTTCGTCTCAGTCTCCCACAAGTCGTACGTAGTATAATTGCATACAAAC
TAAAACACGTTTCTATATATATATAAATGCTGGTGCTTCTTTAAAAATATTCAAGTAGAT
TGAAAGAAAGTGAAGGAACACTATTTCTTTTCTGGAATGAGGAGTTTCAGATCATCCATT
CTCTTGGTGCTTCTTAGTCTTATAACAGTTCTGAATGCCACACGAGCTCGTTCTGAGACT
GAGAGCAAGGTAAAGTGTTCTTTTTTTCTCAGACCGAANAAAGAGAATAGGTTATGACTT
ATGACCAAGTTTTGGTTGTGTTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATATCCGCA

Last Updated on 06.12.2018 16:11