Sequences from confirmation amplicon


Confirmation sequences for line 328C10 and hit At1g07990 are shown.
Line ID 328C10
Locus At1g07990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2481731
Primers used for PCR KN27 - 8474, show primer details
   
Sequence Name 25-K103250-0022-328-C10C-8409
GenBank Accession LN497398 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K103250-0022-328-C10C-8409
TTCGGATGAGCCATTTACATTGAATATATCTGCCATGTAGATACCTCTTCATTGTCATCG
TTTCCATATATTTTGTATCTAAATGCTTGGCTTAAGTTATTGGCCAGAGCTGCAACATCG
TAATCCCTGTCTTCCTCATCGCTATCTCTTGTCCTATCTTGTAGGGTAGTTGGGCGCCTA
CACGTAACAATTAGTTTAGGAATTTTTATAACCAAAAAGAGTGAAGATAAAATCCAAAAC
CCGGGACCAGATCATGGTGCATTTACTGAGTGGTCTCGTCATGAGAAGAAATATTATTTG
ACTATGATACCATCAGTCATCAATGCAGAATTATCTTTGAGGGTTTTGTCCAAGAGGAAC
AAGGATCACACATGAATTTTTACATCCATAATCAGCATTATGTAGCAGACTGACCAATTA
AATGGTTACTAGATGATTTGTGAGTAGAGGTTAACATACCCACATCCCCNTCTGTTGACA
TTTTCAACCGTGTTGCGGTCCTGCAGAACACTACCTTGCCACTCANTCCATTCGCTGTTT
TCCTAANGATTTGGGAATGAACAAAGCACAATCAGCAAACATGGTAGATTCCAACAGAGT
TGCTA
   
Sequence Name 73-K103251-0022-328-C10C-KN27
GenBank Accession LN512877 [GenBank]
Sequencing primer gene specific primer, KN27: 5'-CCTAGCACTCTGTTGGAATCTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K103251-0022-328-C10C-KN27
TCCAATCTTTAGGAAAACAGCGAATGGAATGAGTGGCAAGGTAGTGTTCTGCAGGACCGC
AACACGGTTGAAAATGTCAACAGATGGGGATGTGGGTATGTTAACCTCTACTCACAAATC
ATCTAGTAACCATTTAATTGGTCAGTCTGCTACATAATGCTGATTATGGATGTAAAAATT
CATGTGTGATCCTTGTTCCTCTTGGACAAAACCCTCAAAGATAATTCTGCATTGATGACT
GATGGTATCATAGTCAAATAATATTTCTTCTCATGACGAGACCACTCAGTAAATGCACCA
TGATCTGGTCCCGGGTTTTGGATTTTATCTTCACTCTTTTTGGTTATAAAAATTCCTAAA
CTAATTGTTACGTGTAGGCGCCCAACTACCCTACAAGATNGGACAAGAGATAGCGATAAG
GAAGACNGGGATTACGATGTTGCAGCTCTGGCCAATAACTTAAGCCAAGCATTTAGATAC
AAAATATATGGAAACGATAACNATGAAGAGGTATCTACNTGGCAGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACNTGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
Primers used for PCR KN28 - 8474, show primer details
   
Sequence Name 07-K103729-0022-328-C10C-KN28
GenBank Accession LN489238 [GenBank]
Sequencing primer gene specific primer, KN28: 5'-TATTTTGAGAGACGTCATCATTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K103729-0022-328-C10C-KN28
TAATTCATCACCAGATACAGTAACTGTAGTTTAAAGCAAGGACATGAGGACACCAGTATT
AACAGATAATAAAAAGATAAACTCACCTTCCTTGGTCATCCCCAAGTCTTAAGGATGAAA
TTACTACTTCAGCAGATTCATCGTCGAAGTAAACATCCTGTATAGTATATAAACACATTC
CAATTTTCAATAGCAAACTCTTATTAGCGTACATCCCCCATACGTATCATAGTGTGATAA
AAGATGTTGATAGTTTCCTTGATTTTTCAAAAAAGCGGAGAGAAGAATTTTTGCAAGACT
TACATCATCGTCTCTATCAAGGGCATTGTGGTCCTGAGAGAAATACAAAGACGATAAATT
CAGTACCCAGGAAATCATTCATAAATTGAAGAAAAAGCACTACATGCAGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 23-K103730-0022-328-C10C-8409
GenBank Accession LN496522 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K103730-0022-328-C10C-8409
GCTTTTTCTTCATTTATGAATGATTTCCTGGGTACTGAATTTATCGTCTTTGTATTTCTC
TCAGGACCACAATGCCCTTGATAGAGACGATGATGTAAGTCTTGCAAAAATTCTTCTCTC
CGCTTTTTTGAAAAATCAAGGAAACTATCAACATCTTTTATCACACTATGATACGTATGG
GGGATGTACGCTAATAAGAGTTTGCTATTGAAAATTGGAATGTGTTTATATACTATACAG
GATGTTTACTTCGACGATGAATCTGCTGAAGTAGTAATTTCATCCTTAAGACTTGGGGAT
GACCAAGGAAGGTGAGTTTATCTTTTTATTATCTGTTAATACTGGTGTCCTCATGTCCTT
GCTTTAAACTACAGTTACTGTATCTTGGTGATTGAATTATGTCATCTCTCTTGACCAAAA
AACGAATGATGACGTCCCTCAAAAT

Last Updated on 06.12.2018 16:11