Sequences from confirmation amplicon


Confirmation sequences for line 328D02 and hit At5g64960 are shown.
Line ID 328D02
Locus At5g64960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25957062
Primers used for PCR 11hg - 8474, show primer details
   
Sequence Name 36-K112479-0022-328-D02C-11hg
GenBank Accession LN502280 [GenBank]
Sequencing primer gene specific primer, 11hg: 5'-CATTTTCTCAAGTAGTTCAAGGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K112479-0022-328-D02C-11hg
TGAGATCTCCTGGATCAAGTAACTGTTGCATAAAAGAGTCGCAACTACATAATGGTTTAT
ACTTACTGTCTGTAGATCTCCCTTACGCGTCTCTTTAAGGGCCGAGATGATTTCATTTGG
TTATACCAAGGCATCTTGGAAACTCCAGGCCAGTTGCTTTCATCAGGTGATCCACAAAGC
TCATAAATCTTATTCAATTGTTCATTCTGGAAAGAAAAAAATATGACTAAAACGCTAAGT
GGAAACATCATCAAAGAACACTGTAATAACACACACCACGATAATCATAAGATGAAATCC
TGACCTCAGTTTTCCCAGGCAAGATTGGTTTTCCATTCAAAAGTTCAGCAAATATGCATC
CAACTGACCACATGTCAATCGCTGGTCCATATTTAGTTGCACCAAGTAACAATTCAGGGG
GCCTACAGCAATAAACAATGCATAAGTAGAGAAAAAGCTGCACGTATAAAAAGAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCACGATTAT
   
Sequence Name 06-K112478-0022-328-D02C-8409
GenBank Accession LN488881 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K112478-0022-328-D02C-8409
ATTTCTTTTTATCGTGCAGCTTTTTCTCTACTTATGCATTGTTTATTGCTGTAGGCCCCC
TGAATTGTTACTTGGTGCAACTAAATATGGACCAGCGATTGACATGTGGTCAGTTGGATG
CATATTTGCTGAACTTTTGAATGGAAAACCAATCTTGCCTGGGAAAACTGAGGTCAGGAT
TTCATCTTATGATTATCGTGGTGTGTGTTATTACAGTGTTCTTTGATGATGTTTCCACTT
AGCGTTTTAGTCATATTTTTTTCTTTCCAGAATGAACAATTGAATAAGATTTATGAGCTT
TGTGGATCACCTGATGAAAGCAACTGGCCTGGAGTTTCCAAGATGCCTTGGTATAACCAA
ATGAAATCATCTCGGCCCTTAAAGAGACGCGTAAGGGAGATCTACAGACAGTAAGTATAA
ACCATTATGTAGTTGCGACTCTTTTATGCAACAGTTACTTTGATCCAGGAGATCTCATTT
GGTCCTTTTCTCTTCTGCTATAGCTTTGATCGCCATGCCCTTGAACTACTTGAGAAAATG

Last Updated on 06.12.2018 16:11