Sequences from confirmation amplicon


Confirmation sequences for line 328H06 and hit At5g48810 are shown.
Line ID 328H06
Locus At5g48810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19789637
Primers used for PCR GD81 - 8474, show primer details
   
Sequence Name 67-K033709-022-328-H06C-GD81
GenBank Accession LN511745 [GenBank]
Sequencing primer gene specific primer, GD81: 5'-TCGTACACAAGCAATTCACTTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K033709-022-328-H06C-GD81
CCTTGGTCTTAGTGTAGTACCGAATGCCGAAAGCCAAGCCTAAGATTAGAAGTGGAACAA
GGAACTGAAGGAGCTTAATAACAAAATCCGAGCTCTTATCCTGAGTAGCCACGGCTTTCG
TCGACGTAGGAGGCACAAACTTAGCTTTAACCGGCACAGTAGCTGTGTCAATATCACCCA
CATAGTACTCATCTAGCATGGCTTTCGCAGTCGAACTATGTCCCACATCCTCGAAATCAT
CGGTCGCATCTTTCCCTGAATACCAACAACACATTCTTGTTAACATAAACATGAACTAAC
AAACAGAGAAAGTGATCTCTATATGTTACCTGTAGAAGTCAAGATAACCTCATCACCACC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCACTCCCAAAATGG
   
Sequence Name 51-K033709-022-328-H06C-oriL
GenBank Accession LN507412 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K033709-022-328-H06C-oriL
GCCATTTACATTGAATATATCCTGGTGGTGATGAGGTTATCTTGACTTCTACAGGTAACA
TATAGAGATCACTTTCTCTGTTTGTTAGTTCATGTTTATGTTAACAAGAATGTGTTGTTG
GTATTCAGGGAAAGATGCGACCGATGATTTCGAGGATGTGGGACATAGTTCGACTGCGAA
AGCCATGCTAGATGAGTACTATGTGGGTGATATTGACACAGCTACTGTGCCGGTTAAAGC
TAAGTTTGTGCCTCCTACGTCGACGAAAGCCGTGGCTACTCAGGATAAGAGCTCGGATTT
TGTTATTAAGCTCCTTCAGTTCCTTGTTCCACTTCTAATCTTAGGCTTGGCTTTCGGCAT
TCGGTACTACACTAAGACCAAGGCTCCTTCTTCTTGAAAGATTGAATTGTGGCCTAAGTG
AATTGCTTGTGTACGA
Primers used for PCR GD82 - 8474, show primer details
   
Sequence Name 68-K033709-022-328-H06C-GD82
GenBank Accession LN511943 [GenBank]
Sequencing primer gene specific primer, GD82: 5'-TATCTTCGTGAGATCCGAAATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K033709-022-328-H06C-GD82
AGTTATGTAGTAACAAGTTTGGGGATAGATCTGATCTGATTTGCTAGATTAATGTTACTT
TTCTAAGTCTAAATCACAGAGAAATTGCTAAAAACTTACGTCTTTATAATTGAATGTGAT
AGTTGGTGCTGCTTAAGATGTCTCTGTGTGATCTTGTATCTGTAGTTAAAGCTAGTAAGG
TTCATGTAGTTCATGTATGTTCTGATGTATGAACTTTTGATAACCCACTTAATCTAGGTC
TCTGATTGTGTGGAATTAGGGACTTATATGACAAAGTTTACATTTTTTGTGTGTTTCATT
TTGATGGTTATATATAGGTTTATGATGTGACAAAGTTCTTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 52-K033709-022-328-H06C-8409
GenBank Accession LN507728 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K033709-022-328-H06C-8409
GCCATTTACAGAACTTTGTCACATCATAAACCTATATATAACCATCACAATGAAACACAC
AAAAAATGTAAACTTTGTCATATAAGTCCCTAATTCCACACAATCAGAGACCTAGATTAA
GTGGGTTATCAAAAGTTCATACATCAGAACATACATGAACTACATGAACCTTACTAGCTT
TAACTACAGATACAAGATCACACAGAGACATCTTAAGCAGCACCAACTATCACATTCAAT
TATAAAGACGTAAGTTTTTAGCAATTTCTCTGTGATTTAGACTTAGAAAAGTAACATTAA
TCTAGCAAATCAGATCAGATCTATCCCCAAACTTGTTACTACATAACTCTAGATTTATAA
ATCCAAGTCAACACAACTCAAGAATTTCGGATCTCACGAAGAT

Last Updated on 06.12.2018 16:11