Sequences from confirmation amplicon


Confirmation sequences for line 329F09 and hit At5g15630 are shown.
Line ID 329F09
Locus At5g15630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5086054
Primers used for PCR PC70 - 8474, show primer details
   
Sequence Name 73-K103791-0022-329-F09C-PC70
GenBank Accession LN512884 [GenBank]
Sequencing primer gene specific primer, PC70: 5'-AAAGCCTTGCACATCTCTAATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K103791-0022-329-F09C-PC70
CTTTTTTCTTTACATCTTTGCATGAGTTTACAAAAAACAGTTCTGGTTCAGTGTTTTAAA
ACCCAGAAGGATCAGATCAAATCACCATATTGAGATGAATAGGAGAAGAAGAATGGTGAG
TGAGAACGAAGCAAAGTTCCCTTGTGCAGAGTTTGGTAGAAAAGGGTATGAATCTGGTGG
AGGTAACATACATTCATCACCATTAAAGTAAACTTTTCTAGGAAAAGCCCAACCTTGCTT
GAAAGTAAAAGTCTTTTGATCTTTCTGTAGCAAAACCTCTGATTGCACATTCCCTGAAGG
TCCAGCTTCCATTAATAAATCATTGTAAAACTTCGTTCCATAGAACATTCCAGTATCATC
TGTATCAAAAAGATTTAAGAATGTTATTAGTCATTCAATGGTTGGTTTGAAGTTGAATAG
TTGTGGTTGAGGGAGGTATTTATTTACTTATGGATCCGTAAGGAGAGACTGGTTTGTAGT
CAAAGCTGAAAACTTGAGTCACATTGTTGAGATTTGGATGCTGAATTGCTAAAGTCCAGA
GTGTATGATTCATCCGGTAATTGAAATTTGTGAATGGCACATGTCCGGACATGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCGGTATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCTTTTCAAAAAA
   
Sequence Name 89-K103792-0022-329-F09C-8409
GenBank Accession LN514948 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K103792-0022-329-F09C-8409
GCCATTTACATGTCCGGACATGTGCCATTCACAAATTTCAATTACCGGATGAATCATACA
CTCTGGACTTTAGCAATTCAGCATCCAAATCTCAACAATGTGACTCAAGTTTTCAGCTTT
GACTACAAACCAGTCTCTCCTTACGGATCCATAAGTAAATAAATACCTCCCTCAACCACA
ACTATTCAACTTCAAACCAACCATTGAATGACTAATAACATTCTTAAATCTTTTTGATAC
AGATGATACTGGAATGTTCTATGGAACGAAGTTTTACAATGATTTATTAATGGAAGCTGG
ACCTTCAGGGAATGTGCAATCAGAGGTTTTGCTACAGAAAGATCAAAAGACTTTTACTTT
CAAGCAAGGTTGGGCTTTTCCTAGAAAAGTTTACTTTAATGGTGATGAATGTATGTTACC
TCCACCAGATTCATACCCTTTTCTACCAAACTCTGCACAAGGGAACTTTGCTTCGTTCTC
ACTCACCATTCTTCTTCTCCTATTCATCTCAATATGGTGATTTGATCTGATCCTTCTGGG
TTTTAAAACACTGAACCAGAACTGTTTTTTGTAAACTCATTGCAAAGATTGTAAAAGAAA
AAAAGGAGTTTTGTGAGAGGAAATGATTAGAGATGTGCAAGGCT

Last Updated on 06.12.2018 16:11